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编码人GM2激活蛋白的两个cDNA克隆的证据。

Evidence for two cDNA clones encoding human GM2-activator protein.

作者信息

Nagarajan S, Chen H C, Li S C, Li Y T, Lockyer J M

机构信息

Department of Biochemistry, Tulane University School of Medicine, New Orleans, LA 70112.

出版信息

Biochem J. 1992 Mar 15;282 ( Pt 3)(Pt 3):807-13. doi: 10.1042/bj2820807.

DOI:10.1042/bj2820807
PMID:1554364
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1130859/
Abstract

Two cDNAs encoding GM2 activator, pGM2A (648 bp) and GAP (1093 bp), were isolated from human placenta lambda gt11 libraries. The DNA sequence of pGM2A from 1 to 302 was almost identical with GAP, but diverged from 303-648. PCR was used to demonstrate the presence of both species of GM2 activator in placental RNA. Both cDNAs hybridized to mRNAs of approximately 2.3 kb and to identical single bands on genomic Southern blots.

摘要

从人胎盘λgt11文库中分离出两个编码GM2激活剂的cDNA，即pGM2A（648 bp）和GAP（1093 bp）。pGM2A从1到302的DNA序列与GAP几乎相同，但在303 - 648处出现分歧。采用PCR技术证实胎盘RNA中存在这两种GM2激活剂。两种cDNA均与约2.3 kb的mRNA杂交，并在基因组Southern印迹上与相同的单一条带杂交。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f24/1130859/47093f9a9bea/biochemj00139-0192-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f24/1130859/5e3547977be7/biochemj00139-0191-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f24/1130859/ebc8c3421ddd/biochemj00139-0191-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f24/1130859/47093f9a9bea/biochemj00139-0192-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f24/1130859/5e3547977be7/biochemj00139-0191-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f24/1130859/ebc8c3421ddd/biochemj00139-0191-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f24/1130859/47093f9a9bea/biochemj00139-0192-a.jpg

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本文引用的文献

1
A protein activator for the enzymic hydrolysis of GM2 ganglioside.一种用于GM2神经节苷脂酶促水解的蛋白质激活剂。
J Biol Chem. 1981 Jun 25;256(12):6234-40.
2
Biosynthesis and catabolism of glycosphingolipids.
Adv Carbohydr Chem Biochem. 1982;40:235-88. doi: 10.1016/s0065-2318(08)60110-9.
3
A protein activator of galactosylceramide beta-galactosidase.半乳糖神经酰胺β-半乳糖苷酶的一种蛋白质激活剂。
Biochim Biophys Acta. 1982 Sep 14;712(3):639-49. doi: 10.1016/0005-2760(82)90293-4.
利用圆二色光谱和荧光光谱研究GM2激活蛋白与GM2的相互作用。
Neurochem Res. 2002 Aug;27(7-8):785-92. doi: 10.1023/a:1020204907261.
4
Structure of the GM2A gene: identification of an exon 2 nonsense mutation and a naturally occurring transcript with an in-frame deletion of exon 2.GM2A基因的结构：外显子2无义突变的鉴定以及一种外显子2框内缺失的天然转录本。
Am J Hum Genet. 1999 Jul;65(1):77-87. doi: 10.1086/302463.
5
Complete localization of disulfide bonds in GM2 activator protein.GM2激活蛋白中二硫键的完全定位
Protein Sci. 1998 Apr;7(4):1039-45. doi: 10.1002/pro.5560070421.
6
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Biochem J. 1993 Aug 15;294 ( Pt 1)(Pt 1):227-30. doi: 10.1042/bj2940227.
7
New nucleotide sequence data on the EMBL File Server.欧洲分子生物学实验室文件服务器上的新核苷酸序列数据。
Nucleic Acids Res. 1992 Jun 25;20(12):3257-75. doi: 10.1093/nar/20.12.3257.
4
The protein activator specific for the enzymic hydrolysis of GM2 ganglioside in normal human brain and brains of three types of GM2 gangliosidosis.正常人脑及三种类型GM2神经节苷脂沉积症患者脑内对GM2神经节苷脂进行酶促水解的特异性蛋白质激活剂。
J Neurochem. 1983 Jan;40(1):168-75. doi: 10.1111/j.1471-4159.1983.tb12667.x.
5
Deficiency of the hexosaminidase A activator protein in a case of GM2 gangliosidosis; variant AB.GM2神经节苷脂贮积症变异型AB一例中己糖胺酶A激活蛋白缺乏
Pediatr Res. 1982 Mar;16(3):217-22. doi: 10.1203/00006450-198203000-00011.
6
beta-Glucosidase activator protein from bovine spleen ("coglucosidase").来自牛脾脏的β-葡萄糖苷酶激活蛋白（“伴葡萄糖苷酶”）
Arch Biochem Biophys. 1981 Apr 15;208(1):248-60. doi: 10.1016/0003-9861(81)90147-8.
7
A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
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8
Efficient isolation of genes by using antibody probes.使用抗体探针高效分离基因。
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8. doi: 10.1073/pnas.80.5.1194.
9
Complexing of glycolipids and their transfer between membranes by the activator protein for degradation of lysosomal ganglioside GM2.糖脂的复合作用及其通过溶酶体神经节苷脂GM2降解激活蛋白在膜之间的转移。
Eur J Biochem. 1982 Apr 1;123(2):455-64. doi: 10.1111/j.1432-1033.1982.tb19789.x.
10
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.变性RNA与转移至硝酸纤维素膜上的小DNA片段的杂交。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5. doi: 10.1073/pnas.77.9.5201.