Bellachioma G, Stirling J L, Orlacchio A, Beccari T
Dipartimento di Medicina Sperimentale e Scienze Biochimiche, Universita di Perugia, Italy.
Biochem J. 1993 Aug 15;294 ( Pt 1)(Pt 1):227-30. doi: 10.1042/bj2940227.
A cDNA (1.1 kb) containing the complete coding sequence for the mouse GM2 activator protein was isolated from a mouse macrophage library using a cDNA for the human protein as a probe. There was a single ATG located 12 bp from the 5' end of the cDNA clone followed by an open reading frame of 579 bp. Northern blot analysis of mouse macrophage RNA showed that there was a single band with a mobility corresponding to a size of 2.3 kb. We deduce from this that the mouse mRNA, in common with the mRNA for the human GM2 activator protein, has a long 3' untranslated sequence of approx. 1.7 kb. Alignment of the mouse and human deduced amino acid sequences showed 68% identity overall and 75% identity for the sequence on the C-terminal side of the first 31 residues, which in the human GM2 activator protein contains the signal peptide. Hydropathicity plots showed great similarity between the mouse and human sequences even in regions of low sequence similarity. There is a single N-glycosylation site in the mouse GM2 activator protein sequence (Asn151-Phe-Thr) which differs in its location from the single site reported in the human GM2 activator protein sequence (Asn63-Val-Thr).
使用人GM2激活蛋白的cDNA作为探针，从小鼠巨噬细胞文库中分离出一个包含小鼠GM2激活蛋白完整编码序列的cDNA（1.1 kb）。在cDNA克隆的5'端12 bp处有一个单一的ATG，其后是一个579 bp的开放阅读框。对小鼠巨噬细胞RNA的Northern印迹分析表明，有一条迁移率对应于2.3 kb大小的单带。由此我们推断，小鼠mRNA与人类GM2激活蛋白的mRNA一样，具有约1.7 kb的长3'非翻译序列。小鼠和人类推导的氨基酸序列比对显示，总体同一性为68%，前31个残基C端一侧的序列同一性为75%，在人类GM2激活蛋白中该区域包含信号肽。亲水性图谱显示，即使在序列相似性较低的区域，小鼠和人类序列之间也有很大的相似性。小鼠GM2激活蛋白序列中有一个单一的N-糖基化位点（Asn151-Phe-Thr），其位置与人类GM2激活蛋白序列中报道的单个位点（Asn63-Val-Thr）不同。
