Rahman Nafisur, Singh Manisha, Hoda Md Nasrul
Analytical Research Division, Department of Chemistry, Aligarh Muslim University, Aligarh, 202002 UP, India.
Farmaco. 2004 Nov;59(11):913-9. doi: 10.1016/j.farmac.2004.07.009.
Three new spectrophotometric methods for the determination of amlodipine besylate have been proposed. The first two methods, i.e. A and B, are based on the oxidation of the drug with Fe(III) and the estimation of Fe(II) produced after chelation with either 1,10-phenanthroline or 2,2'-bipyridyl at 500 and 515 nm, respectively. The Beer's law was obeyed in the concentration ranges of 2-10 and 4-14 microg ml(-1) with molar absorptivity of 2.9 x 10(4) and 2.7 x 10(4) l mol(-1) cm(-1) for methods A and B, respectively. The third procedure depends on the interaction of amlodipine besylate with ammonium heptamolybdate tetrahydrate, which resulted in the formation of molybdenum blue (lambda(max) 825 nm). The linear dynamic range and the molar absorptivity values were found to be 15-59 microg ml(-1) and 1.8 x 10(4) l mol(-1) cm(-1), respectively. The results of the proposed procedures were validated statistically and compared with those obtained by the reference method. The proposed methods were applied successfully to the determination of amlodipine besylate in commercial tablets.
已提出三种测定苯磺酸氨氯地平的新分光光度法。前两种方法，即方法A和方法B，基于药物与Fe(III)的氧化反应，并分别通过与1,10 - 菲啰啉或2,2'-联吡啶螯合后，在500和515 nm处测定生成的Fe(II)。方法A和方法B在2 - 10和4 - 14 μg ml(-1)的浓度范围内符合比尔定律，摩尔吸光系数分别为2.9×10(4)和2.7×10(4) l mol(-1) cm(-1)。第三种方法依赖于苯磺酸氨氯地平与四水合七钼酸铵的相互作用，这导致形成钼蓝（最大吸收波长825 nm）。线性动态范围和摩尔吸光系数值分别为15 - 59 μg ml(-1)和1.8×10(4) l mol(-1) cm(-1)。所提出方法的结果经统计学验证，并与参考方法获得的结果进行比较。所提出的方法已成功应用于市售片剂中苯磺酸氨氯地平的测定。
