Kristensen K, Bentzon M W
Streptococcus Department, Statens Seruminstitut, Copenhagen, Denmark.
APMIS. 1992 Feb;100(2):142-6. doi: 10.1111/j.1699-0463.1992.tb00853.x.
The measurement of antibodies to the capsular polysaccharide (PRP) of Haemophilus influenzae type b (Hib) is important because vaccines inducing such antibodies are now available. We developed and evaluated an enzyme-linked immunosorbent assay (ELISA) for detection of these antibodies based on direct coating of the plates with tyraminated PRP. The assay fulfilled the requirements for parallel line assays; it was sensitive, specific, and reproducible with a coefficient of variation between days of 19%. Results from the ELISA were compared with results from radioimmunoassay and a correlation coefficient of 0.93 was found. Results obtained by the two methods were proportional and the relation was independent of the antibody level. The relation between them was also unaffected by the contribution of different antibody isotypes, indicating that these were measured to the same extent by both methods. ELISA employing direct coating of the plates with tyraminated PRP represents a useful alternative for detection of antibodies when studying immunogenicity of Hib vaccines.
对b型流感嗜血杆菌（Hib）荚膜多糖（PRP）抗体的检测很重要，因为现在已有诱导此类抗体的疫苗。我们开发并评估了一种基于用酪胺化PRP直接包被酶标板来检测这些抗体的酶联免疫吸附测定（ELISA）。该测定满足平行线测定的要求；它灵敏、特异且可重复，不同日期间的变异系数为19%。将ELISA的结果与放射免疫测定的结果进行比较，发现相关系数为0.93。两种方法获得的结果成比例，且这种关系与抗体水平无关。它们之间的关系也不受不同抗体同种型贡献的影响，这表明两种方法对它们的测量程度相同。当研究Hib疫苗的免疫原性时，采用酪胺化PRP直接包被酶标板的ELISA是检测抗体的一种有用替代方法。
