Zhang Jianli, Liu Zhiheng, Goodfellow Michael
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, People's Republic of China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2301-2305. doi: 10.1099/ijs.0.63133-0.
The taxonomic position of a soil isolate, strain 276(T), was established using a polyphasic approach. The organism showed a range of chemical and morphological properties consistent with its classification in the genus Nocardia. An almost complete 16S rRNA gene sequence determined for the strain was aligned with corresponding sequences of representatives of the genus Nocardia and related taxa using three tree-making algorithms. The organism formed a distinct phyletic line within the evolutionary radiation occupied by the genus Nocardia and was most closely related to the type strain of Nocardia abscessus. However, the two strains shared a low DNA-DNA relatedness value and were readily distinguished using a combination of phenotypic properties. The combined genotypic and phenotypic data show that strain 276(T) should be assigned to the genus Nocardia as a novel species. The name proposed for this new taxon is Nocardia xishanensis sp. nov. The type strain is 276(T) (=CGMCC 4.1860(T)=JCM 12160(T)).
采用多相分类法确定了土壤分离株276(T)的分类地位。该菌株表现出一系列化学和形态学特性，与其在诺卡氏菌属中的分类相符。使用三种建树算法，将该菌株测定的近乎完整的16S rRNA基因序列与诺卡氏菌属及相关分类单元代表的相应序列进行比对。该菌株在诺卡氏菌属所占据的进化辐射区内形成了一条独特的系统发育线，与脓肿诺卡氏菌模式菌株关系最为密切。然而，这两个菌株的DNA-DNA相关性值较低，并且通过表型特性组合很容易区分。基因型和表型数据综合表明，菌株276(T)应归为诺卡氏菌属的一个新物种。为这个新分类单元提议的名称是西山诺卡氏菌（Nocardia xishanensis sp. nov.）。模式菌株是276(T)（=CGMCC 4.1860(T)=JCM 12160(T)）。
