Zhang Jianli, Liu Zhiheng, Goodfellow Michael
State Key Laboratory of Microbial Resource, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Department of Agricultural and Environmental Science, University of Newcastle, Newcastle-upon-Tyne NE1 7RU, UK.
Int J Syst Evol Microbiol. 2003 Jul;53(Pt 4):999-1004. doi: 10.1099/ijs.0.02397-0.
A soil isolate, strain F829T, which had provisionally been assigned to the genus Nocardia, was subjected to a polyphasic taxonomic study. An almost complete 16S rDNA sequence was determined for this strain; the sequence was aligned with available sequences for nocardiae and phylogenetic trees were inferred using three tree-making algorithms. The organism showed a combination of phenotypic properties typical of nocardiae and formed a distinct phyletic line within the evolutionary radiation of species of the genus Nocardia, being most closely related to Nocardia asteroides ATCC 19247T. Strain F829T was readily distinguished from representatives of species of Nocardia with validly published names on the basis of phenotypic data, notably from the type strain of N. asteroides. It is proposed that the organism be recognized as a novel species of Nocardia, Nocardia caishijiensis sp. nov. The type strain is F829T (=AS 4.1728T=JCM 11508T).
一株曾被暂定为诺卡氏菌属的土壤分离菌株F829T，进行了多相分类研究。测定了该菌株几乎完整的16S rDNA序列；将该序列与诺卡氏菌的现有序列进行比对，并使用三种建树算法推断系统发育树。该菌表现出诺卡氏菌典型的一系列表型特征，在诺卡氏菌属物种的进化辐射范围内形成了一个独特的进化谱系，与星形诺卡氏菌ATCC 19247T关系最为密切。基于表型数据，菌株F829T很容易与已有效发表名称的诺卡氏菌属物种的代表区分开来，特别是与星形诺卡氏菌的模式菌株区分开来。建议将该菌认定为诺卡氏菌属的一个新物种，即采石矶诺卡氏菌（Nocardia caishijiensis sp. nov.）。模式菌株为F829T（=AS 4.1728T=JCM 11508T）。
