Branger Judith, Wieland Catharina W, Florquin Sandrine, Maris Nico A, Pater Jennie M, Speelman Peter, Shimizu Takao, Ishii Satoshi, van der Poll Tom
Department of Experimental Internal Medicine, Academic Medical Center, University of Amsterdam, The Netherlands.
Shock. 2004 Dec;22(6):543-7. doi: 10.1097/01.shk.0000142818.91693.73.
Platelet-activating factor (PAF), a glycerophospholipid with proinflammatory properties, exerts its biological effects by interacting with the PAF receptor (PAFR) expressed on many different cell types. The PAFR specifically binds phosphorylcholine, the biologically active component of PAF. However, phosphorylcholine is also a component of the cell wall of nontypeable Haemophilus influenzae (NTHi). In recently published in vitro experiments, the invasion of respiratory epithelial cells by NTHi was mediated by the PAFR. To determine the role of the PAFR in host defense against pneumonia induced by NTHi, PAFR-deficient (PAFR-/-) and normal wild-type mice were intranasally inoculated with NTHi. The absence of a functional PAFR was associated with a normal innate immune response as indicated by similar bacterial counts, myeloperoxidase activity, and inflammation within the pulmonary compartment of PAFR-/- and wild-type mice. These data indicate that the PAFR does not interfere with the clearance of NTHi from the respiratory tract.
血小板活化因子（PAF）是一种具有促炎特性的甘油磷脂，它通过与多种不同细胞类型上表达的PAF受体（PAFR）相互作用来发挥其生物学效应。PAFR特异性结合PAF的生物活性成分磷酰胆碱。然而，磷酰胆碱也是不可分型流感嗜血杆菌（NTHi）细胞壁的组成成分。在最近发表的体外实验中，NTHi对呼吸道上皮细胞的侵袭是由PAFR介导的。为了确定PAFR在宿主抵御NTHi诱导的肺炎中的作用，将PAF受体缺陷（PAFR-/-）小鼠和正常野生型小鼠经鼻接种NTHi。PAFR-/-小鼠和野生型小鼠肺内细菌计数、髓过氧化物酶活性及炎症反应相似，表明缺乏功能性PAFR与正常的固有免疫反应相关。这些数据表明，PAFR不会干扰呼吸道中NTHi的清除。
