基于微卫星DNA和AFLP标记，利用香鱼（Plecoglossus altivelis）纯合克隆鱼进行连锁图谱构建的初步研究。
Preliminary study on linkage mapping based on microsatellite DNA and AFLP markers using homozygous clonal fish in ayu (Plecoglossus altivelis).
作者信息
Watanabe Tomohisa, Fujita Hironobu, Yamasaki Kimio, Seki Shingo, Taniguchi Nobuhiko
机构信息
Laboratory of Applied Population Genetics, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-amamiyamachi, Sendai, Miyagi 981-8555, Japan.
出版信息
Mar Biotechnol (NY). 2004 Jul-Aug;6(4):327-34. doi: 10.1007/s10126-003-0026-4. Epub 2004 Jun 23.
A mapping referential family (F(1)) of ayu was produced by crossing a normal diploid male with a homozygous clonal female. A genetic linkage map was constructed using 191 amplified fragment length polymorphism (AFLP) and 4 microsatellite DNA markers. A total of 178 loci were mapped in 36 linkage groups comprising 1659.6 cM, which includes approximately 77.3% to 81.8% of the total genome. As the markers were randomly distributed over the genome, they showed high efficiency for the construction of a wide linkage map.
通过将正常二倍体雄性与纯合克隆雌性杂交，构建了香鱼的一个映射参考家系（F(1)）。利用191个扩增片段长度多态性（AFLP）和4个微卫星DNA标记构建了遗传连锁图谱。总共178个位点被定位到36个连锁群中，覆盖长度为1659.6 cM，约占基因组总量的77.3%至81.8%。由于这些标记在基因组上随机分布，它们在构建广泛的连锁图谱方面显示出高效率。
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