Cervera M T, Storme V, Ivens B, Gusmão J, Liu B H, Hostyn V, Van Slycken J, Van Montagu M, Boerjan W
Vakgroep Moleculaire Genetica en Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, Ledeganckstraat 35, B-9000 Gent, Belgium.
Genetics. 2001 Jun;158(2):787-809. doi: 10.1093/genetics/158.2.787.
Populus deltoides, P. nigra, and P. trichocarpa are the most important species for poplar breeding programs worldwide. In addition, Populus has become a model for fundamental research on trees. Linkage maps were constructed for these three species by analyzing progeny of two controlled crosses sharing the same female parent, Populus deltoides cv. S9-2 x P. nigra cv. Ghoy and P. deltoides cv. S9-2 x P. trichocarpa cv. V24. The two-way pseudotestcross mapping strategy was used to construct the maps. Amplified fragment length polymorphism (AFLP) markers that segregated 1:1 were used to form the four parental maps. Microsatellites and sequence-tagged sites were used to align homoeologous groups between the maps and to merge linkage groups within the individual maps. Linkage analysis and alignment of the homoeologous groups resulted in 566 markers distributed over 19 groups for P. deltoides covering 86% of the genome, 339 markers distributed over 19 groups for P. trichocarpa covering 73%, and 369 markers distributed over 28 groups for P. nigra covering 61%. Several tests for randomness showed that the AFLP markers were randomly distributed over the genome.
美洲黑杨、欧洲黑杨和毛果杨是全球杨树育种计划中最重要的树种。此外，杨树已成为树木基础研究的模式植物。通过分析两个共享同一母本美洲黑杨品种S9 - 2的受控杂交后代，即美洲黑杨品种S9 - 2×欧洲黑杨品种Ghoy和美洲黑杨品种S9 - 2×毛果杨品种V24，构建了这三个树种的连锁图谱。采用双向伪测交作图策略构建图谱。分离比例为1:1的扩增片段长度多态性（AFLP）标记用于构建四个亲本图谱。微卫星和序列标签位点用于比对各图谱间的同源群，并合并单个图谱内的连锁群。连锁分析和同源群比对结果显示，美洲黑杨有566个标记分布在19个连锁群上，覆盖基因组的86%；毛果杨有339个标记分布在19个连锁群上，覆盖73%；欧洲黑杨有369个标记分布在28个连锁群上，覆盖61%。多项随机性检验表明，AFLP标记在基因组上随机分布。
