Standop Jens, Schneider Matthias, Ulrich Alexis, Mathiak Guenther, Brand Randall E, Buechler Markus W, Pour Parviz M
UNMC Eppley Cancer Center, University of Nebraska Medical Center, Omaha, NE, USA.
Oncol Rep. 2004 Dec;12(6):1309-15.
Histological differentiation between chronic pancreatitis and pancreatic cancer, especially in biopsy material, remains challenging and the frequent association of 'secondary' chronic pancreatitis (due to ductal obstruction) with pancreatic cancer causes additional diagnostic problems. Our study, using anti-ErbB2 antibodies from Santa Cruz and Dako in tissues from the normal pancreas, chronic pancreatitis and pancreatic cancer showed that these antibodies discriminate between primary chronic pancreatitis and 'secondary' chronic pancreatitis due to pancreatic cancer. Tissues from 28 pancreatic cancer patients, 15 chronic pancreatitis patients and 12 organ donors or early autopsy cases were subjected to immunohistochemical studies using polyclonal ErbB2 antibodies from Santa Cruz and Dako. The Santa Cruz antibody immunoreacted with islet cells in all tissues from the normal pancreas and pancreatic cancer but not in any chronic pancreatitis specimen. The Dako antibody showed a membrane staining of ductal and ductular cells only in chronic pancreatitis cases but in none of the normal or cancer specimens. Moreover, in chronic pancreatitis cases, ductular cells were stained with the Santa Cruz antibody only in the severe form, but not in the mild or moderate form of the disease. The utilized ErbB2 antibodies discriminate between the normal pancreas, chronic pancreatitis and pancreatic cancer. Hence, these antibodies seem to present an additional useful aid in the surgical pathology of pancreatic diseases.
慢性胰腺炎与胰腺癌之间的组织学鉴别，尤其是在活检材料中，仍然具有挑战性，并且“继发性”慢性胰腺炎（由于导管阻塞）与胰腺癌的频繁关联引发了额外的诊断问题。我们的研究使用来自圣克鲁斯（Santa Cruz）和达科（Dako）的抗ErbB2抗体，对正常胰腺、慢性胰腺炎和胰腺癌组织进行检测，结果显示这些抗体能够区分原发性慢性胰腺炎和由胰腺癌引起的“继发性”慢性胰腺炎。对28例胰腺癌患者、15例慢性胰腺炎患者以及12例器官捐献者或早期尸检病例的组织，使用来自圣克鲁斯和达科的多克隆ErbB2抗体进行免疫组化研究。圣克鲁斯抗体在正常胰腺和胰腺癌的所有组织中的胰岛细胞中均有免疫反应，但在任何慢性胰腺炎标本中均无反应。达科抗体仅在慢性胰腺炎病例中显示导管和小导管细胞的膜染色，而在正常或癌症标本中均未显示。此外，在慢性胰腺炎病例中，仅在疾病的严重形式中，小导管细胞被圣克鲁斯抗体染色，而在轻度或中度形式中则未染色。所使用的ErbB2抗体能够区分正常胰腺、慢性胰腺炎和胰腺癌。因此，这些抗体似乎为胰腺疾病的外科病理学提供了一种额外的有用辅助手段。
