Zhang Kai-Li, Ma Jing-Xin, Chen Xiao-Yan, Sun Yuan, Kong Qing-You, Liu Jia, Li Hong
Cancer Institute and Laboratory of Cell Biology and Molecular Genetics, College of Basic Medical Sciences, Dalian Medical University, Dalian 116027, PR China.
Oncol Rep. 2004 Dec;12(6):1335-40.
CYP1A1 expression in various tissues of 43 gastric cancer cases was analyzed with frozen tissue array-based immunohistochemical staining (IHC), RT-PCR, Western blot analysis and EROD (7-ethoxyresourfin-O-deethylase) assay. CYP1A1 was detected immunohistochemically in 86% (37/43) of gastric cancers, in both 57% (4/7) of atrophic gastritis and intestinal metaplasia and in 7.7% (1/13) of non-cancerous mucosa. RT-PCR and Western blot analysis revealed coincident results with that of IHC and ruled out possible concurrent CYP1A2 expression. EROD assay showed increased CYP1A1/1B1 activity in either cancer or premalignant tissues but not in non-cancerous ones. The frequencies of CYP1A1 expression are significantly different between non-cancerous and premalignant (P<0.025) or cancer groups (P<0.005), suggesting that CYP1A1 is expressed at relatively early stage of gastrocarcinogenesis and exerts its effects throughout the stepwise oncogenic processes.
采用基于冰冻组织芯片的免疫组织化学染色（IHC）、逆转录-聚合酶链反应（RT-PCR）、蛋白质免疫印迹分析及7-乙氧基异吩恶唑酮-O-脱乙基酶（EROD）活性检测法，分析43例胃癌患者各组织中细胞色素P450 1A1（CYP1A1）的表达情况。免疫组织化学检测显示，86%（37/43）的胃癌组织中可检测到CYP1A1，57%（4/7）的萎缩性胃炎及肠化生组织和7.7%（1/13）的非癌黏膜组织中也可检测到。RT-PCR及蛋白质免疫印迹分析结果与免疫组织化学检测结果一致，且排除了同时存在细胞色素P450 1A2（CYP1A2）表达的可能性。EROD活性检测显示，CYP1A1/1B1活性在癌组织或癌前组织中升高，而在非癌组织中未升高。非癌组织与癌前组织（P<0.025）或癌组织（P<0.005）之间CYP1A1表达频率差异有统计学意义，提示CYP1A1在胃癌发生的相对早期即开始表达，并在整个肿瘤发生的逐步过程中发挥作用。
