Dimassi N, Bril A, Autissier N, Bralet J, Rochette L
Facultés de Medicine et Pharmacie, Dijon, France.
Cardioscience. 1992 Mar;3(1):7-12.
The relation between myocardial norepinephrine and reperfusion arrhythmias was examined in isolated rat hearts. The influence of pre-treatment with reserpine on the incidence of reperfusion arrhythmias and on the accumulation of calcium in the myocardium during ischemia and reperfusion was also studied. Ischemia was induced by ligation of the coronary artery and, in some experiments, the cardiac stores of norepinephrine were labeled with 3H-norepinephrine. Reperfusion after an ischemic period of 10 or 15 minutes induced a marked release of radioactivity and reperfusion arrhythmias. The radioactivity liberated after ischemia appeared to have been trapped during ischemia and washed out during reperfusion. Pre-treatment of the hearts with 0.1 mg/kg reserpine 24 hours before the experiment protected against reperfusion arrhythmias and significantly reduced the accumulation of calcium during reperfusion. The results confirm that the nature of reperfusion arrhythmias is highly dependent upon the duration of the ischemic period and sympathetic activity.
在离体大鼠心脏中研究了心肌去甲肾上腺素与再灌注心律失常之间的关系。还研究了利血平预处理对再灌注心律失常发生率以及缺血和再灌注期间心肌钙积累的影响。通过结扎冠状动脉诱导缺血，在一些实验中，用3H-去甲肾上腺素标记心肌中的去甲肾上腺素储备。缺血10或15分钟后的再灌注诱导了放射性的显著释放和再灌注心律失常。缺血后释放的放射性似乎在缺血期间被捕获，并在再灌注期间被冲洗掉。在实验前24小时用0.1mg/kg利血平预处理心脏可预防再灌注心律失常，并显著减少再灌注期间的钙积累。结果证实，再灌注心律失常的性质高度依赖于缺血期的持续时间和交感神经活动。
