Sonoki Tomonori, Kajita Shinya, Ikeda Seiichiro, Uesugi Mikiko, Tatsumi Kenji, Katayama Yoshihiro, Iimura Yosuke
Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology, 16-1 Onogawa, Tsukuba, Ibaraki 305-0053, Japan.
Appl Microbiol Biotechnol. 2005 Apr;67(1):138-42. doi: 10.1007/s00253-004-1770-8. Epub 2004 Nov 12.
The phytoremediation of soils contaminated with organic pollutants offers a low-cost method for removal of such pollutants. We have attempted to enhance the environmental decontamination functions of plants by introducing appropriate enzymatic activities from microorganisms. In the present study, we introduced an extracellular fungal enzyme, the laccase of Coriolus versicolor, into tobacco plants. One transgenic plant, designated FL4, produced laccase that was secreted into the rhizosphere. FL4 was able to remove 20 micromol bisphenol A or pentachlorophenol per gram dry weight. The efficiency of this removal was apparently greater than that of control lines. Our results should stimulate efforts to develop plant-based technologies for the removal of environmental pollutants from contaminated environments.
对受有机污染物污染的土壤进行植物修复，为去除此类污染物提供了一种低成本的方法。我们试图通过引入来自微生物的适当酶活性来增强植物的环境净化功能。在本研究中，我们将一种细胞外真菌酶——云芝漆酶，导入烟草植株。一种名为FL4的转基因植物产生了分泌到根际的漆酶。FL4每克干重能够去除20微摩尔双酚A或五氯苯酚。这种去除效率明显高于对照品系。我们的研究结果应会激励人们努力开发基于植物的技术，以从受污染环境中去除环境污染物。
