Brown Allison S, Reid Angela D, Leamen Lisa, Cucevic Viviene, Foster F Stuart
Imaging Research, Sunnybrook Health Sciences Centre, Toronto, ONT, Canada.
Ultrasound Med Biol. 2004 Sep;30(9):1223-32. doi: 10.1016/j.ultrasmedbio.2004.07.020.
Little has been reported on bioeffects of high-frequency ultrasound (US) and guidelines for US use do not necessarily apply to high frequencies. Pregnant CD-1 mice were exposed to Doppler or B-mode US biomicroscopy (UBM) on embryonic day (E) 8.5 or E10.5, during organogenesis. Operating frequency was 40 MHz with a free field I(SPTA) of 11.9 W/cm(2) (Doppler) and 2.6 mW/cm(2) (B-mode), peak negative pressures of 6.61 MPa and MI of 1.05 (B-mode). Offspring were assessed weekly from 1 day postnatally to euthanasia at 6 weeks, with no significant difference in pup weight, body length or crown-rump length observed. E8.5 Doppler-exposed mice showed a small reduction in weight and length at 3 weeks and in weight at 6 weeks. E10.5 Doppler-exposed animals exhibited slight growth reduction in weeks 2 to 4, but were not significantly different at 6 weeks. Our results indicate similar exposures of mice should not cause significant adverse bioeffects.
关于高频超声（US）的生物效应报道较少，且超声使用指南不一定适用于高频情况。在器官形成期的胚胎第（E）8.5天或E10.5天，对怀孕的CD-1小鼠进行多普勒或B型超声生物显微镜检查（UBM）。操作频率为40MHz，自由场声强空间峰值时间平均声强I(SPTA)为11.9W/cm²（多普勒）和2.6mW/cm²（B型），峰值负压为6.61MPa，机械指数为1.05（B型）。从出生后1天到6周安乐死期间，每周对后代进行评估，未观察到幼崽体重、体长或顶臀长有显著差异。E8.5天接受多普勒超声照射的小鼠在3周时体重和体长略有下降，6周时体重下降。E10.5天接受多普勒超声照射的动物在第2至4周生长略有减缓，但在6周时无显著差异。我们的结果表明，对小鼠进行类似照射不应引起显著的不良生物效应。
