大鼠心脏肥大细胞分离过程中的组胺自发分泌。
Spontaneous histamine secretion during isolation of rat cardiac mast cells.
作者信息
Forman M F, Brower G L, Janicki J S
机构信息
Department of Anatomy, Physiology and Pharmacology, Auburn University, Auburn, AL 36849-5517, USA.
出版信息
Inflamm Res. 2004 Sep;53(9):453-7. doi: 10.1007/s00011-004-1275-z.
OBJECTIVE
To test the hypothesis that spontaneous release of histamine occurring during an isolation protocol may modify responses of rat cardiac mast cells (connective tissue-type mast cells) to secretagogues.
METHODS
We assessed two protocols for enzymatic dispersion utilizing collagenase, hyaluronidase, and deoxyribonuclease; with protease (Protocol 1, n = 8) or without protease (Protocol 2, n = 3). Spontaneous release of histamine was quantified following mechanical and enzymatic dispersion of the whole heart.
RESULTS
Total histamine loss (Mean +/- SEM) was 963+/-92 and 833+/-60 ng/g of tissue weight following Protocols 1 and 2. Percentages of histamine release from cell isolates following Protocol 1 were 40+/-5%, 41+/-6%, and 51+/-7% at 0, 30, and 300 microg/mL of compound 48/80.
CONCLUSIONS
Enzymatic dispersion of cardiac mast cells affects their response to secretagogues.
目的
验证在分离过程中组胺的自发释放可能会改变大鼠心脏肥大细胞（结缔组织型肥大细胞）对促分泌剂反应的这一假设。
方法
我们评估了两种使用胶原酶、透明质酸酶和脱氧核糖核酸酶进行酶解分散的方案；一种添加蛋白酶（方案1，n = 8），另一种不添加蛋白酶（方案2，n = 3）。在对整个心脏进行机械和酶解分散后，对组胺的自发释放进行定量。
结果
按照方案1和方案2，组织重量中组胺的总损失（平均值±标准误）分别为963±92和833±60 ng/g。在方案1中，化合物48/80浓度为0、30和300 μg/mL时，细胞分离物中组胺的释放百分比分别为40±5%、41±6%和51±7%。
结论
心脏肥大细胞的酶解分散会影响其对促分泌剂的反应。
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