Soll A H, Lewin K, Beaven M A
Gastroenterology. 1979 Dec;77(6):1283-90.
The cells of the fundic portion of canine gastric mucosa were dispersed by collagenase digestion and separated into fractions by sequential use of velocity sedimentation in an elutriator rotor followed by a density gradient separation. There was a close correlation between histamine content and number of mast cells in the different cell fractions. The mast cells possessed characteristic dense granules, which stained metachromatically, but did not release histamine on exposure to Compound 48/80. The most highly purified fractions contained 80% mast cells and a histamine content of 2.5 pg/mast cell.
犬胃黏膜胃底部的细胞经胶原酶消化后分散，通过在淘析转子中依次进行速度沉降，随后进行密度梯度分离，将其分离成不同组分。不同细胞组分中组胺含量与肥大细胞数量之间存在密切相关性。肥大细胞具有特征性的致密颗粒，可进行异染性染色，但暴露于化合物48/80时不释放组胺。纯化程度最高的组分含有80%的肥大细胞，组胺含量为2.5 pg/肥大细胞。
