Venkataraman P, Sridhar M, Dhanammal Siva, Vijayababu M R, Arunkumar A, Srinivasan N, Arunakaran J
Department of Endocrinology, Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani, Chennai, India.
Endocr Res. 2004 Aug;30(3):469-80. doi: 10.1081/erc-200035959.
The objective of this study was to determine the impact of the polychlorinated biphenyl (PCB) Aroclor 1254 and vitamin C and E on ventral prostatic testosterone and estradiol receptor concentration. A group of 30 rats were treated with Aroclor 1254 [2 mg/kg body weight (bwt) /day/ip] for 30 days; 10 rats were treated as Aroclor 1254 control. The remaining 20 rats were subdivided into the following two subgroups of 10 animals each. One group was given vitamin C (500 mg/kg bwt/day/oral) for 10 days, whereas the other group was given vitamin E (50 mg/kg bwt/day/oral) for 10 days. Separate controls were also maintained. Ventral prostatic androgen and estrogen receptor concentration in all the groups were quantified. Serum hormonal profiles such as total T3, T4, TSH, testosterone, and estradiol were also estimated in all the groups. Ventral prostatic zinc content and serum zinc concentration were also determined in all the groups. Aroclor 1254 exposure decreased the concentration of both receptors. Decreased serum total T3, T4, testosterone, estradiol, and increased TSH were observed after Aroclor 1254 exposure. Serum and tissue zinc levels were also decreased. Administration of vitamin C or E restored both the receptor concentration and the serum hormone levels with the exception of estradiol. Administration of vitamin C or E restored zinc levels. Vitamin E was more sensitive on ventral prostatic androgen receptors and zinc levels, including serum, in PCB-exposed rats.
本研究的目的是确定多氯联苯（PCB）Aroclor 1254以及维生素C和E对前列腺腹侧睾酮和雌二醇受体浓度的影响。一组30只大鼠接受Aroclor 1254 [2毫克/千克体重（bwt）/天/腹腔注射]处理30天；10只大鼠作为Aroclor 1254对照组。其余20只大鼠被分成两个亚组，每组10只动物。一组给予维生素C（500毫克/千克bwt/天/口服），持续10天，而另一组给予维生素E（50毫克/千克bwt/天/口服），持续10天。同时设立单独的对照组。对所有组的前列腺腹侧雄激素和雌激素受体浓度进行定量。还对所有组的血清激素谱，如总T3、T4、促甲状腺激素（TSH）、睾酮和雌二醇进行了评估。还测定了所有组的前列腺腹侧锌含量和血清锌浓度。暴露于Aroclor 1254会降低两种受体的浓度。暴露于Aroclor 1254后，观察到血清总T3、T4、睾酮、雌二醇降低，TSH升高。血清和组织锌水平也降低。给予维生素C或E可恢复受体浓度和血清激素水平，但雌二醇除外。给予维生素C或E可恢复锌水平。在暴露于PCB的大鼠中，维生素E对前列腺腹侧雄激素受体和包括血清在内的锌水平更为敏感。
