利用原子力显微镜对修饰云母表面上的单链DNA长分子进行纳米操纵。

Nanomanipulation of extended single-DNA molecules on modified mica surfaces using the atomic force microscopy.

作者信息

Lü Jun Hong

机构信息

Nanobiology and Nanomedicine Laboratory, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.

出版信息

Colloids Surf B Biointerfaces. 2004 Dec 25;39(4):177-80. doi: 10.1016/j.colsurfb.2004.07.013.

DOI:10.1016/j.colsurfb.2004.07.013

PMID:15555900

Abstract

The technique of nanometer scale manipulation is very important in constructing nano-structures and nano-devices. By using atomic force microscope, three kinds of controllable manipulation on single-DNA molecules were introduced in the paper. DNA molecules deposited and extended on modified mica surface were first imaged by tapping mode, then cutting, bending, and pushing were respectively performed on single-DNA molecules. The results of the manipulation depend on the interaction between tip and DNA as well as between substrate and DNA.

摘要

纳米尺度操纵技术在构建纳米结构和纳米器件方面非常重要。本文利用原子力显微镜介绍了对单链DNA分子的三种可控操纵。首先用轻敲模式对沉积并伸展在修饰云母表面的DNA分子进行成像，然后分别对单链DNA分子进行切割、弯曲和推压操作。操纵结果取决于针尖与DNA之间以及基底与DNA之间的相互作用。