用于原子力显微镜的云母化学处理会影响生物样品构象。

Chemical treatment of mica for atomic force microscopy can affect biological sample conformation.

作者信息

Costa Lilian T, Pinto José R, Moraes Marta B, de Souza Guttierre G B, Sorenson Martha M, Bisch Paulo M, Weissmüller Gilberto

机构信息

Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21949-900, Brazil.

出版信息

Biophys Chem. 2004 Apr 1;109(1):63-71. doi: 10.1016/j.bpc.2003.10.011.

DOI:10.1016/j.bpc.2003.10.011

PMID:15059660

Abstract

An important aspect in the preparation of substrate materials to use in atomic force microscopy lies in the question of interactions introduced by treatments designed to immobilize the sample over the substrate. Here we used a mica substrate that was chemically modified with cationic nickel to immobilize actin filaments (F-actin). Chemical modification could be followed quantitatively by measuring the interaction force between the scanning tip and the mica surface. This approach allowed us to observe polymeric F-actin in a structure that resembles an actin gel. It also improved sample throughput and conferred sample stability as well as repeatability from run to run.

摘要

用于原子力显微镜的基底材料制备中的一个重要方面在于，为将样品固定在基底上而进行的处理所引入的相互作用问题。在这里，我们使用了用阳离子镍进行化学修饰的云母基底来固定肌动蛋白丝（F-肌动蛋白）。通过测量扫描探针与云母表面之间的相互作用力，可以对化学修饰进行定量跟踪。这种方法使我们能够观察到类似于肌动蛋白凝胶结构的聚合F-肌动蛋白。它还提高了样品通量，赋予了样品稳定性以及每次运行之间的可重复性。