Ohishi Kazue, Kishida Noriko, Ninomiya Ai, Kida Hiroshi, Takada Yoshitake, Miyazaki Nobuyuki, Boltunov Andrei N, Maruyama Tadashi
Research Program for Marine Biology and Ecology, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Kanagawa.
Microbiol Immunol. 2004;48(11):905-9. doi: 10.1111/j.1348-0421.2004.tb03610.x.
Antibodies to influenza A virus were detected using enzyme-linked immunosorbent assay (ELISA) in the sera from two of seven Baikal seals (Phoca sibrica) and from five of six ringed seals (Phoca hispida) in Russia. In a hemagglutination-inhibition test using H1-H15 reference influenza A viruses, ELISA-positive sera from one Baikal seal and four ringed seals reacted to A/Aichi/2/68 (H3N2) and A/Bangkok/1/79 (H3N2) strains. One ringed seal serum sample reacted to A/seal/Massachusetts/1/80 (H7N7). The present results suggested that human-related H3 viruses were prevalent in Baikal seals and ringed seals inhabiting the central Russian Arctic.
采用酶联免疫吸附测定(ELISA)法,在俄罗斯贝加尔湖海豹(西伯海豹)7只中的2只以及环斑海豹6只中的5只血清中检测到了甲型流感病毒抗体。在使用H1-H15甲型流感参考病毒进行的血凝抑制试验中,1只贝加尔湖海豹和4只环斑海豹的ELISA阳性血清与A/爱知/2/68(H3N2)和A/曼谷/1/79(H3N2)毒株发生反应。1份环斑海豹血清样本与A/海豹/马萨诸塞州/1/80(H7N7)发生反应。目前的结果表明,与人相关的H3病毒在栖息于俄罗斯北极中部的贝加尔湖海豹和环斑海豹中普遍存在。