Oliveira R C, Rozental T, Alves-Corrêa A A, D'Andrea P S, Schatzmayr H G, Cerqueira R, Lemos E R S
Laboratório de Hantaviroses e Rickettsioses, Departamento de Virologia, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz-Fiocruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brazil.
Mem Inst Oswaldo Cruz. 2004 Oct;99(6):575-6. doi: 10.1590/s0074-02762004000600007. Epub 2004 Nov 18.
Wild sigmondontine rodents are known to be the reservoir of several serotypes of New World hantaviruses. The mechanism of viral transmission is by aerosol inhalation of the excreta from infected rodents. Considering that the captive breed colonies of various wild mammals may present a potential risk for hantaviral transmission, we examined 85 specimens of Thrichomys spp. (Echimyidae) and 17 speciemens of Nectomys squamipes (Sigmodontinae) from our colony for the presence of hantavirus infections. Blood samples were assayed for the presence of antibodies to Andes nucleocapsid antigen using enzyme-linked immunosorbent assay (ELISA). Additionally, serum samples from workers previously exposed to wild rodents, in the laboratories where the study was conducted, were also tested by ELISA to investigate prevalence of anti-hantavirus IgG antibodies. All blood samples were negative for hantavirus antibodies. Although these results suggest that those rodent's colonies are hantavirus free, the work emphasizes the need for hantavirus serological monitoring in wild colonized rodents and secure handling potentially infected rodents as important biosafety measures.
野生稻鼠科啮齿动物是多种新大陆汉坦病毒血清型的宿主。病毒传播机制是通过吸入受感染啮齿动物排泄物的气溶胶。鉴于各种野生哺乳动物的圈养繁殖群体可能存在汉坦病毒传播的潜在风险，我们检测了来自我们养殖群体的85份粗尾鼠属（棘鼠科）标本和17份鳞尾木鼠（稻鼠亚科）标本，以检测是否存在汉坦病毒感染。使用酶联免疫吸附测定（ELISA）检测血样中是否存在针对安第斯核衣壳抗原的抗体。此外，在进行该研究的实验室中，还对之前接触过野生啮齿动物的工作人员的血清样本进行了ELISA检测，以调查抗汉坦病毒IgG抗体的流行情况。所有血样的汉坦病毒抗体均为阴性。尽管这些结果表明那些啮齿动物群体没有汉坦病毒，但这项工作强调了对野生圈养啮齿动物进行汉坦病毒血清学监测以及安全处理潜在感染啮齿动物作为重要生物安全措施的必要性。
