Sibikeeva Iu E, Sibikeev S N, Krupnov V A
Genetika. 2004 Sep;40(9):1224-8.
Leaf-rust resistance and androgenesis were studied in the anther cultures of Triticum aestivum L., which included Saratovskaya 29 cultivar, the isogenic line Ps29, and three F1 hybrids (L503/S55, L504/S58, ATS7/L1063) with 7DS-7DL-7Ae#1L translocation of Lr19 gene (Lr19 translocation) from Agropyron elongatum (Host) P.B. The Lr19 translocation was shown to affect the induction of embryogenesis and green plant regeneration. The frequencies of Lr19 translocation differed in F2 hybrids obtained by traditional hybridization and in sets of DH lines obtained in F1 anther cultures derived from the same combinations of T. aestivum parental forms. The number of leaf-rust resistant genotypes tended to decrease. The frequency of Lr19 translocation in the set of DH3 lines derived from F1 L504/S58 was significantly lower than in other sets of DH3 lines and F2 hybrid populations.
在普通小麦(Triticum aestivum L.)的花药培养中研究了叶锈病抗性和雄核发育,其中包括萨拉托夫斯卡娅29品种、同基因系Ps29以及三个具有来自长穗偃麦草(Agropyron elongatum (Host) P.B.)的Lr19基因7DS - 7DL - 7Ae#1L易位(Lr19易位)的F1杂种(L503/S55、L504/S58、ATS7/L1063)。结果表明,Lr19易位影响胚胎发生诱导和绿色植株再生。通过传统杂交获得的F2杂种以及由普通小麦亲本形式相同组合的F1花药培养获得的双单倍体系中,Lr易位19的频率不同。抗叶锈病基因型的数量趋于减少。来自F1 L504/S58的DH3系组中Lr19易位的频率显著低于其他DH3系组和F2杂交群体。