Sibikeeva Yu E, Sibikeev S N
Genetika. 2014 Jul;50(7):831-9.
The features of in vitro androgenesis were studied in Cultured anthers of spring bread wheats L503 and Dobrynya, having 7DS-7DL-7Ae#1 L translocation with genes Lrl9/Sr25 (Lrl9 translocation) from Agropyron elongatum (Host.) P.B. and their near-isogenic lines carrying combinations of Lrl9 translocation with translocations: 1BL-IR#1S with genes Pm8/Sr31/Lr26/Yr9 (Lr26translocation) from Secale cereal L., 4BS-4BL-2R#1L with genes Lr25/Pm7 (Lr25 translocation) from Secale cereal, 3DS-3DL-3Ae#1L with genes Lr24/Sr24 (Lr24 translocation) from Agropyron elongatum and 6BS-6BL-6U#1L with gene Lr9 (Lr9 translocation) from Aegilops umbellulata Zhuk. In comparison with those varieties having received the Lrl9 translocation, the following was established: (1) the combination of translocations Lr19+26 increased embryo frequency and green plant regeneration; (2) the combination of translocations Lr19+9 decreased embryo frequency but increased green plant regeneration; (3) the combination of translocations Lr19+24 decreased embryo frequency but increased green and albino plant regeneration; (4) the combination of translocations Lr19+25 increased embryo frequency and green plant regeneration but decreased albino plant regeneration. Thus, on near-isogenic lines of spring bread wheat, the influences of genotypes of four alien translocation combinations on in vitro androgenesis were determined.
对春小麦L503和Dobrynya的离体花药进行了研究，这两个品种具有来自长穗偃麦草（Host.）P.B.的带有Lrl9/Sr25基因（Lrl9易位）的7DS - 7DL - 7Ae#1L易位，以及它们携带Lrl9易位与以下易位组合的近等基因系：来自黑麦的带有Pm8/Sr31/Lr26/Yr9基因（Lr26易位）的1BL - IR#1S、来自黑麦的带有Lr25/Pm7基因（Lr25易位）的4BS - 4BL - 2R#1L、来自长穗偃麦草的带有Lr24/Sr24基因（Lr24易位）的3DS - 3DL - 3Ae#1L以及来自伞穗山羊草朱克的带有Lr9基因（Lr9易位）的6BS - 6BL - 6U#1L。与接受Lrl9易位的那些品种相比，确定了以下情况：（1）Lr19 + 26易位组合增加了胚频率和绿色植株再生；（2）Lr19 + 9易位组合降低了胚频率但增加了绿色植株再生；（3）Lr19 + 24易位组合降低了胚频率但增加了绿色和白化植株再生；（4）Lr19 + 25易位组合增加了胚频率和绿色植株再生但降低了白化植株再生。因此，在春小麦的近等基因系上确定了四种外源易位组合的基因型对离体雄核发育的影响。
