Kurtböke D I, Chen C F, Williams S T
University of Liverpool, Department of Genetics and Microbiology, UK.
J Appl Bacteriol. 1992 Feb;72(2):103-11. doi: 10.1111/j.1365-2672.1992.tb01810.x.
An isolation method was developed in which prior to inoculation soil suspensions were exposed to suspensions of polyvalent phage isolated to Streptomyces spp. The phage susceptibility of streptomycetes provided a selective means of reducing streptomycetes on isolation plates subsequent to inoculation, and this reduction was persistent after long incubation periods. The efficiency and applicability of the method developed were checked with different samples from a range of sources. The increased chances of development of other genera after the reduction of streptomycetes on soil dilution plates were assessed.
开发了一种分离方法,即在接种前将土壤悬浮液暴露于分离自链霉菌属的多价噬菌体悬浮液中。链霉菌的噬菌体敏感性提供了一种在接种后减少分离平板上链霉菌的选择性方法,并且在长时间培养后这种减少仍然持续。用来自一系列来源的不同样品检查了所开发方法的效率和适用性。评估了土壤稀释平板上链霉菌减少后其他属发育机会增加的情况。