Al Hamad Bader M, Al Raish Seham M, Ramadan Gaber A, Saeed Esam Eldin, Alameri Shaikha S A, Al Senaani Salima S, AbuQamar Synan F, El-Tarabily Khaled A
Department of Biology, College of Science, United Arab Emirates University, Al Ain 15551, United Arab Emirates.
Hemaya Institute for Health, Safety, Environment and Food Science, Sharjah Research Technology and Innovation Park, Sharjah 66636, United Arab Emirates.
J Fungi (Basel). 2021 Oct 20;7(11):885. doi: 10.3390/jof7110885.
To manage stem canker disease on royal poinciana, actinobacterial isolates were used as biological control agents (BCAs) based on their strong inhibitory effects against . UAE2 and UAE1 had the ability to produce antifungal compounds and cell-wall-degrading enzymes (CWDEs). Only , however, restored the activity of 1-aminocyclopropane-1-carboxylate (ACC) deaminase (ACCD). apple fruit bioassay showed that lesion development was successfully constrained by either isolates on fruits inoculated with . In our greenhouse and container nursery experiments, showed almost complete suppression of disease symptoms. This was evident when the preventive treatment of significantly ( < 0.05) reduced the numbers of conidia of and defoliated leaves of royal poinciana seedlings to lesser levels than when was applied, but comparable to control treatments (no pathogen). The disease management of stem canker was also associated with significant ( < 0.05) decreases in ACC levels in royal poinciana stems when was applied compared to the non-ACCD-producing . This study is the first to report the superiority of antagonistic actinobacteria to enhance their effectiveness as BCAs not only for producing antifungal metabolites and CWDEs but also for secreting ACCD.
为了防治凤凰木的茎溃疡病，基于放线菌分离株对……具有强烈抑制作用，将其用作生物防治剂（BCAs）。阿联酋2号和阿联酋1号能够产生抗真菌化合物和细胞壁降解酶（CWDEs）。然而，只有……恢复了1-氨基环丙烷-1-羧酸（ACC）脱氨酶（ACCD）的活性。苹果果实生物测定表明，在接种了……的果实上，两种分离株都成功抑制了病斑发展。在我们的温室和容器苗圃试验中，……几乎完全抑制了病害症状。当……的预防性处理显著（P<0.05）降低凤凰木幼苗上……的分生孢子数量和落叶数量，使其低于施用……时的水平，但与对照处理（无病原菌）相当，这一点很明显。与不产生ACCD的……相比，施用……时，凤凰木茎中ACC水平也显著（P<0.05）降低，茎溃疡病的病害管理也与之相关。本研究首次报道了拮抗性放线菌作为生物防治剂的优势，即不仅能产生抗真菌代谢产物和CWDEs，还能分泌ACCD，从而提高其有效性。
