高通量筛选技术：利用斑马鱼的现状与未来

Technology for high-throughput screens: the present and future using zebrafish.

作者信息

Love Donald R, Pichler Franz B, Dodd Andrew, Copp Brent R, Greenwood David R

机构信息

School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1001, New Zealand.

出版信息

Curr Opin Biotechnol. 2004 Dec;15(6):564-71. doi: 10.1016/j.copbio.2004.09.004.

DOI:10.1016/j.copbio.2004.09.004

PMID:15560983

Abstract

The zebrafish is a popular vertebrate model organism with similar organ systems and gene sequences to humans. Zebrafish embryos are optically transparent enabling organ visualisation, which can be complemented with gene expression analysis at the transcript and protein levels. Furthermore, zebrafish can be treated with small molecules and drugs in a microtitre plate format for high-throughput analysis and for the identification and validation of drugs. High-throughput methodologies for use in zebrafish include phenotype-based visualisation, transcript studies using low-density DNA microarrays and proteomic analysis. These technologies offer significant whole-organism biological value in the drug discovery and drug development pipeline.

摘要

斑马鱼是一种广受欢迎的脊椎动物模式生物，其器官系统和基因序列与人类相似。斑马鱼胚胎具有光学透明性，便于观察器官，这可以在转录和蛋白质水平上通过基因表达分析得到补充。此外，斑马鱼可以在微量滴定板中用小分子和药物进行处理，以进行高通量分析以及药物的鉴定和验证。用于斑马鱼的高通量方法包括基于表型的可视化、使用低密度DNA微阵列的转录研究和蛋白质组学分析。这些技术在药物发现和药物开发流程中具有重要的全生物体生物学价值。