Kang Bijun, Cai Yizuo, Jia Zhuoxuan, Yao Liudi, Gu Jiancheng, Li Wei, Zhang Wenjie
Department of Plastic and Reconstructive Surgery, Shanghai 9thPeople's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, National Tissue Engineering Center of China, 639 ZhiZaoJu Road, Shanghai, 200011, China.
SEME Cell Technology Co., Ltd, 68 East Jiangchuan Road, Shanghai, 200241, China.
Aesthetic Plast Surg. 2025 Mar;49(5):1526-1535. doi: 10.1007/s00266-024-04445-9. Epub 2024 Nov 12.
The formation of infraorbital dark circles involves various factors including hyperpigmentation, skin aging and laxity. This study is to investigate whether cell-free fat extract (CEFFE) inhibits melanin synthesis and improves the appearance of infraorbital dark circles.
Imaging systems analysed the melanin content in three groups of zebrafish embryos treated with 0 (CTRL), 100, and 200μg/mL of CEFFE. Eleven patients with infraorbital dark circles were enrolled and received intradermal injections of autologous CEFFE. Clinical trial assessments included subjective assessments (Global Aesthetic Improvement Scale and Likert satisfaction scale) and objective assessments (standard two-dimensional photographs, VISIA® photographs, and infraorbital skin colour measurement).
In the experiment of zebrafish embryos, the melanin content of CEFFE treatment was significantly reduced. In clinical trial, 72.73% of patients considered CEFFE treatment for infraorbital dark circles to be effective, and more than half of the patients were satisfied with the treatment. In the evaluation of skin colour at the inner, middle, and outer edges of the infraorbital dark circles, there was a gradual improvement in infraorbital skin colour after each treatment and at the 3-month follow-up, with a statistically significant improvement in skin colour on the outer edge of the infraorbital dark circles (p < 0.05). Simultaneously, we observed a reduction in periocular wrinkles, with a significant difference at the 3-month follow-up (p < 0.05). The events observed included bruising, erythema, and oedema, which resolved spontaneously.
Our study demonstrated that CEFFE inhibits melanogenesis and is a safe and effective treatment for infraorbital dark circles.
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眶下黑眼圈的形成涉及多种因素，包括色素沉着、皮肤老化和松弛。本研究旨在探讨无细胞脂肪提取物（CEFFE）是否能抑制黑色素合成并改善眶下黑眼圈外观。
成像系统分析了用0（对照组）、100和200μg/mL CEFFE处理的三组斑马鱼胚胎中的黑色素含量。招募了11名眶下黑眼圈患者，接受自体CEFFE皮内注射。临床试验评估包括主观评估（整体美学改善量表和李克特满意度量表）和客观评估（标准二维照片、VISIA®照片和眶下皮肤颜色测量）。
在斑马鱼胚胎实验中，CEFFE处理组的黑色素含量显著降低。在临床试验中，72.73%的患者认为CEFFE治疗眶下黑眼圈有效，超过一半的患者对治疗满意。在眶下黑眼圈内、中、外边缘的皮肤颜色评估中，每次治疗后及3个月随访时眶下皮肤颜色逐渐改善，眶下黑眼圈外边缘皮肤颜色有统计学显著改善（p < 0.05）。同时，我们观察到眼周皱纹减少，在3个月随访时有显著差异（p < 0.05）。观察到的事件包括瘀斑、红斑和水肿，均自行消退。
我们的研究表明，CEFFE可抑制黑色素生成，是治疗眶下黑眼圈的一种安全有效的方法。
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