利用免疫球蛋白Fc片段受体对人β溶血性G群链球菌进行菌种鉴别
Differentiation of species in human beta-haemolytic group G streptococci using immunoglobulin Fc fragment receptor.
作者信息
Cimolai N, Cheong A C
机构信息
Division of Medical Microbiology, University of British Columbia, Canada.
出版信息
J Clin Pathol. 1992 Mar;45(3):232-4. doi: 10.1136/jcp.45.3.232.
AIMS
To assess the ability of human immunoglobulin Fc fragment binding activity to differentiate human biotype large colony group G streptococci from the group G "Streptococcus milleri group".
METHODS
Fifty two isolates of large colony group G streptococci and 30 group G "S milleri group" strains were tested for their ability to bind fluorescein conjugated human IgG Fc fragments after acetone fixation. Immunoblotting with peroxidase labelled human Fc fragments after resolution of bacterial polypeptides by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) was performed for six large colony strains.
RESULTS
All large colony group G streptococci showed positive Fc fragment binding whereas all "S milleri group" bacteria failed to bind Fc fragments when viewed by fluorescence microscopy. All six large colony strains showed similar immunoblot binding patterns.
CONCLUSION
Immunoglobulin Fc fragment receptor content distinguishes the large colony group G streptococci from the group G "S milleri group" and mayhave a role in the rapid laboratory diagnosis of pharyngeal pathogens.
目的
评估人免疫球蛋白Fc片段结合活性区分人生物型大菌落G群链球菌与G群“米勒链球菌群”的能力。
方法
对52株大菌落G群链球菌分离株和30株G群“米勒链球菌群”菌株在丙酮固定后检测其结合荧光素偶联的人IgG Fc片段的能力。对6株大菌落菌株在通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离细菌多肽后，用辣根过氧化物酶标记的人Fc片段进行免疫印迹分析。
结果
通过荧光显微镜观察，所有大菌落G群链球菌均显示Fc片段结合阳性，而所有“米勒链球菌群”细菌均未结合Fc片段。所有6株大菌落菌株均显示出相似的免疫印迹结合模式。
结论
免疫球蛋白Fc片段受体含量可区分大菌落G群链球菌与G群“米勒链球菌群”，可能在咽部病原体的快速实验室诊断中发挥作用。
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