[高效液相色谱法测定海马体中次黄嘌呤和黄嘌呤的含量]
[Determination of hypoxanthine and xanthine in hippocampus by HPLC method].
作者信息
Zhao Xiaoli, Cui Xiaobing, Di Liuqing, Li Wei
机构信息
Nanjing University of Traditional Chinese Medicine, Nanjing 210029.
出版信息
Zhong Yao Cai. 2002 Oct;25(10):716-7.
OBJECTIVE
A method was established for the determination of hypoxanthine and xanthine in Hippocampus by HPLC.
METHODS
The HPLC system consisted of a Kromasil C18 column (250 mm x 4.6 mm, 5 microm) with methanol-water (2:98, V/V) as the mobile phase. The wavelength of detection was 254 nm.
RESULTS
The linear range of hypoxanthine and xanthine were 29.0-464.0 ng (r = 0.99996) and 24.5-392.0 ng (r = 0.9995) respectively. The average recovery of hypoxanthine was 100.73%, RSD = 1.31% (n = 6). The average recovery of xanthine was 100.54%, RSD = 1.14% (n = 6).
CONCLUSION
This method is convenient and the result is accurate. It can be used for quality control of Hippocampus.
目的
建立一种用高效液相色谱法测定海马中次黄嘌呤和黄嘌呤的方法。
方法
高效液相色谱系统由Kromasil C18柱（250 mm×4.6 mm，5μm）组成，以甲醇-水（2:98，V/V）为流动相。检测波长为254 nm。
结果
次黄嘌呤和黄嘌呤的线性范围分别为29.0 - 464.0 ng（r = 0.99996）和24.5 - 392.0 ng（r = 0.9995）。次黄嘌呤的平均回收率为100.73%，相对标准偏差（RSD）= 1.31%（n = 6）。黄嘌呤的平均回收率为100.54%，RSD = 1.14%（n = 6）。
结论
该方法简便，结果准确。可用于海马的质量控制。
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