[中华大蟾蜍皮肤中蟾毒硫因的测定及华蟾素注射液]
[Determination of bufothionine in skin of Bufo bufo gargarizans and Huachansu injection].
作者信息
Dai Li-ping, Wang Zhi-min, Gao Hui-min, Jiang Xu, Ding Guang-zhi
机构信息
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2007 Feb;32(3):224-6.
OBJECTIVE
To establish a HPLC method for bufothionine in the skin of Bufo bufo gargarizans and Huachansu injection.
METHOD
The samples were separated using a Lichrosob C18 column with CH3CN-H2O (10:90) as mobile phase. Flow rate was at 1.0 mL x min (-1) and the detection wavelength was at 225 nm.
RESULT
The calibration curve of bufothionine was linear over the range of 0.0772-0.4632 microg and the average recovery was 99. 2%. The contents of bufothionine were fluctuated from 36.4-641.8 microg x g(-1) in the skin of Bufo bufo gargarizans and 22.47-33.16 microg x mL(-1) in Huachansu injection, respectively.
CONCLUSION
The contents of bufothionine were greatly different between cultured and wild species. The method was suitable for the quality control of the skin of Bufo bufo gargarizans and its preparation.
目的
建立一种测定中华大蟾蜍皮肤及华蟾素注射液中蟾毒硫磷含量的高效液相色谱法。
方法
采用Lichrosob C18柱，以乙腈-水（10:90）为流动相进行样品分离。流速为1.0 mL·min⁻¹，检测波长为225 nm。
结果
蟾毒硫磷的校准曲线在0.0772 - 0.4632 μg范围内呈线性，平均回收率为99.2%。中华大蟾蜍皮肤中蟾毒硫磷含量在36.4 - 641.8 μg·g⁻¹之间波动，华蟾素注射液中蟾毒硫磷含量在22.47 - 33.16 μg·mL⁻¹之间。
结论
养殖和野生品种中蟾毒硫磷的含量差异很大。该方法适用于中华大蟾蜍皮肤及其制剂的质量控制。
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