Wu Fang-li, Li Ai-zhen, Mao Hui-fang
Zhejiang Yixin Pharmaceutical Co. LTD, Lanxi 321100, China.
Zhongguo Zhong Yao Za Zhi. 2005 Apr;30(7):523-5.
To establish a method for determination of galanthamine in Lycoris radiata.
HPLC separation was carried on a column of ODS (4.6 mm x 150 mm, 5 microm), with the mobile phase of phosphate buffer (pH = 3-4)-methanol (93:7) at the flow rate of 1.0 mL x min(-1), and the detection wavelength was set at 289 nm.
Galanthamine revealed linearity within the range of 3-30 microg x mL(-1) (r = 0.9997), the detection limit was 0.3 ng. The average recovery was 99.5% (RSD = 0.5%).
The method is easy to operate and the results of the determination are accurate, it can be used to evaluate the quality of L. radiata.
建立测定石蒜中加兰他敏的方法。
采用ODS柱（4.6 mm×150 mm，5μm）进行高效液相色谱分离，流动相为磷酸盐缓冲液（pH = 3 - 4）-甲醇（93:7），流速为1.0 mL·min⁻¹，检测波长设定为289 nm。
加兰他敏在3 - 30μg·mL⁻¹范围内呈线性关系（r = 0.9997），检测限为0.3 ng。平均回收率为99.5%（相对标准偏差RSD = 0.5%）。
该方法操作简便，测定结果准确，可用于评价石蒜的质量。
