[无精子症患者附睾及睾丸精子胞浆内注射的结果]

[Outcome of intracytoplasmic injection of epididymal and testicular sperm obtained from azoospermic patients].

作者信息

Wang Junxia, Sun Haixiang, Hu Yali, Wang Bin, Zhang Ningyuan, Chen Hua, Zhu Weidong

机构信息

Center of Reproductive Medicine, the Affiliated Drum Tower Hospital, Nanjing University Medical College, Nanjing, Jiangsu 210008, China.

出版信息

Zhonghua Nan Ke Xue. 2004 Oct;10(10):751-4.

PMID:15562788

Abstract

OBJECTIVE

To review the treatment results of intracytoplasmic injection of epididymal and testicular sperm obtained from 50 azoospermic patients from January 2003 to May 2004.

METHODS

Sperm was retrieved by percutaneous epididymal sperm aspiration (PESA) or testicular sperm extraction (TESE) and intracytoplasmic sperm injection (ICSI) was performed. The rates of successful sperm retrieval, fertilization, implantation and pregnancy were analysed and evaluated.

RESULTS

In the PESA, TESE and ejaculation groups, the number of M II oocytes suitable for ICSI were 286, 360 and 1569 respectively. The difference of fertilization rates among the three groups was not significant (74.8%, 75.2% vs 77.5%, P>0.05). No difference was seen in the implantation and clinical pregnancy rates of the TESE group from the ejaculation group (29.87% vs 29.54%; 48.15% vs 52.60%, P>0.05). However the implantation and clinical pregnancy rates were higher in the PESA group compared with the TESE and ejaculation groups (50.85% vs 29.87%, 29.54%; 68% vs 48.15%, 52.60%, P<0.05). In the PESA group, 17 clinical pregnancies were achieved including 9 ongoing pregnancies, and 6 live deliveries and 2 miscarriages. While in the TESE group, 13 clinical pregnancies were achieved including 4 ongoing pregnancies, 7 live deliveries and 2 miscarriages.

CONCLUSION

ICSI with PESA and TESE is an effective method to treat azoospermia.

摘要

目的

回顾2003年1月至2004年5月间50例无精子症患者经附睾和睾丸获取精子行卵胞浆内单精子注射（ICSI）的治疗结果。

方法

采用经皮附睾精子抽吸术（PESA）或睾丸精子提取术（TESE）获取精子并进行ICSI。分析并评估精子获取成功率、受精率、着床率及妊娠率。

结果

在PESA组、TESE组和射精组中，适合ICSI的MⅡ期卵母细胞数分别为286、360和1569个。三组受精率差异无统计学意义（74.8%、75.2%对77.5%，P>0.05）。TESE组与射精组的着床率和临床妊娠率差异无统计学意义（29.87%对29.54%；48.15%对52.60%，P>0.05）。然而，PESA组的着床率和临床妊娠率高于TESE组和射精组（50.85%对29.87%、29.54%；68%对48.15%、52.60%，P<0.05）。PESA组获得17例临床妊娠，其中9例为持续妊娠，6例活产，2例流产。TESE组获得13例临床妊娠，其中4例为持续妊娠，7例活产，2例流产。