Sukcharoen N, Sithipravej T, Promviengchai S, Chinpilas V, Boonkasemsanti W
Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
J Med Assoc Thai. 1998 Aug;81(8):565-71.
Intracytoplasmic sperm injection (ICSI) has been successfully used to achieve fertilization and pregnancies for patients with extreme oligoastheno-zoospermia using ejaculated sperms or patients with azoospermia using epididymal or testicular sperms. The aim of this study was to compare the fertilization rate after ICSI using ejaculated, epididymal and testicular sperms. Between January and September 1997, 10 azoospermic men underwent percutaneous epididymal sperm aspiration (PESA) or testicular sperm extraction (TESE) to recover sperm for ICSI. A total of 5 PESA cases and 5 TESE cases were performed at the Center for Assisted Reproduction & Embryology. Thirty-one patients performed ICSI using ejaculated sperms during the same period of time were used as a control group. ICSI using ejaculated sperms, epididymal sperms from PESA and testicular sperms from TESE was a highly successful technique, achieving fertilization rates of 78.5 per cent, 83.3 per cent and 80.8 per cent, respectively. Good fertilization rates were achieved without significant differences among the various sperm sources.
卵胞浆内单精子注射（ICSI）已成功用于为重度少弱畸精子症患者使用射出精子实现受精和妊娠，或为无精子症患者使用附睾或睾丸精子实现受精和妊娠。本研究的目的是比较使用射出精子、附睾精子和睾丸精子进行ICSI后的受精率。1997年1月至9月期间，10名无精子症男性接受了经皮附睾精子抽吸术（PESA）或睾丸精子提取术（TESE）以获取精子用于ICSI。辅助生殖与胚胎学中心共进行了5例PESA病例和5例TESE病例。同期31例使用射出精子进行ICSI的患者作为对照组。使用射出精子、PESA获得的附睾精子和TESE获得的睾丸精子进行ICSI是一项非常成功的技术，受精率分别为78.5%、83.3%和80.8%。不同精子来源均获得了良好的受精率，且无显著差异。
