Shimizu Hiroyuki, Thorley Bruce, Paladin Fem Julia, Brussen Kerri Anne, Stambos Vicki, Yuen Lilly, Utama Andi, Tano Yoshio, Arita Minetaro, Yoshida Hiromu, Yoneyama Tetsuo, Benegas Agnes, Roesel Sigrun, Pallansch Mark, Kew Olen, Miyamura Tatsuo
Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
J Virol. 2004 Dec;78(24):13512-21. doi: 10.1128/JVI.78.24.13512-13521.2004.
In 2001, highly evolved type 1 circulating vaccine-derived poliovirus (cVDPV) was isolated from three acute flaccid paralysis patients and one contact from three separate communities in the Philippines. Complete genomic sequencing of these four cVDPV isolates revealed that the capsid region was derived from the Sabin 1 vaccine strain but most of the noncapsid region was derived from an unidentified enterovirus unrelated to the oral poliovirus vaccine (OPV) strains. The sequences of the cVDPV isolates were closely related to each other, and the isolates had a common recombination site. Most of the genetic and biological properties of the cVDPV isolates were indistinguishable from those of wild polioviruses. However, the most recently identified cVDPV isolate from a healthy contact retained the temperature sensitivity and partial attenuation phenotypes. The sequence relationships among the isolates and Sabin 1 suggested that cVDPV originated from an OPV dose given in 1998 to 1999 and that cVDPV circulated along a narrow chain of transmission. Type 1 cVDPV was last detected in the Philippines in September 2001, and population immunity to polio was raised by extensive OPV campaigns in late 2001 and early 2002.
2001年，在菲律宾三个不同社区的三名急性弛缓性麻痹患者及一名接触者中分离出高度进化的1型循环疫苗衍生脊髓灰质炎病毒（cVDPV）。对这四株cVDPV分离株进行全基因组测序发现，衣壳区域源自Sabin 1疫苗株，但大部分非衣壳区域源自一种与口服脊髓灰质炎疫苗（OPV）株无关的未鉴定肠道病毒。这些cVDPV分离株的序列彼此密切相关，且分离株具有一个共同的重组位点。cVDPV分离株的大多数遗传和生物学特性与野生脊髓灰质炎病毒无法区分。然而，最近从一名健康接触者中鉴定出的cVDPV分离株保留了温度敏感性和部分减毒表型。分离株与Sabin 1之间的序列关系表明，cVDPV起源于1998年至1999年接种的一剂OPV，且cVDPV沿着一条狭窄的传播链传播。2001年9月在菲律宾最后一次检测到1型cVDPV，2001年末和2002年初通过广泛的OPV接种活动提高了人群对脊髓灰质炎的免疫力。
