O'Connor David H, McDermott Adrian B, Krebs Kendall C, Dodds Elizabeth J, Miller Jacqueline E, Gonzalez Edna J, Jacoby Timothy J, Yant Levi, Piontkivska Helen, Pantophlet Ralph, Burton Dennis R, Rehrauer William M, Wilson Nancy, Hughes Austin L, Watkins David I
Wisconsin Primate Research Center, Department of Pathology, Laboratoty of Medicine, University of Wisconsin, 1300 University Ave., Madison, WI 53706, USA.
J Virol. 2004 Dec;78(24):14012-22. doi: 10.1128/JVI.78.24.14012-14022.2004.
CD8(+) T lymphocytes (CD8-TL) select viral escape variants in both human immunodeficiency virus and simian immunodeficiency virus (SIV) infections. The frequency of CD8-TL viral escape as well as the contribution of escape to overall virus diversification has not been assessed. We quantified CD8-TL selection in SIV infections by sequencing viral genomes from 35 SIVmac239-infected animals at the time of euthanasia. Here we show that positive selection for sequences encoding 46 known CD8-TL epitopes is comparable to the positive selection observed for the variable loops of env. We also found that >60% of viral variation outside of the viral envelope occurs within recognized CD8-TL epitopes. Therefore, we conclude that CD8-TL selection is the dominant cause of SIV diversification outside of the envelope.
CD8(+) T淋巴细胞（CD8-TL）在人类免疫缺陷病毒和猴免疫缺陷病毒（SIV）感染中均会选择病毒逃逸变体。尚未评估CD8-TL病毒逃逸的频率以及逃逸对整体病毒多样性的贡献。我们通过在安乐死时对35只感染SIVmac239的动物的病毒基因组进行测序，量化了SIV感染中CD8-TL的选择。在此我们表明，对编码46个已知CD8-TL表位的序列的阳性选择与对env可变环观察到的阳性选择相当。我们还发现，病毒包膜外>60%的病毒变异发生在公认的CD8-TL表位内。因此，我们得出结论，CD8-TL选择是包膜外SIV多样性的主要原因。
