Kubota Masaki, Tsuji Masahisa, Nishimoto Mamoru, Wongchawalit Jintanart, Okuyama Masayuki, Mori Haruhide, Matsui Hirokazu, Surarit Rudee, Svasti Jisnuson, Kimura Atsuo, Chiba Seiya
Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Biosci Biotechnol Biochem. 2004 Nov;68(11):2346-52. doi: 10.1271/bbb.68.2346.
Three kinds of alpha-glucosidases, I, II, and III, were purified from European honeybees, Apis mellifera L. In addition, an alpha-glucosidase was also purified from honey. Some properties, including the substrate specificity of honey alpha-glucosidase, were almost the same as those of alpha-glucosidase III. Specific antisera against the alpha-glucosidases were prepared to examine the localization of alpha-glucosidases in the organs of honeybees. It was immunologically confirmed for the first time that alpha-glucosidase I was present in ventriculus, and alpha-glucosidase II, in ventriculus and haemolymph. alpha-Glucosidase III, which became apparent to be honey alpha-glucosidase, was present in the hypopharyngeal gland, from which the enzyme may be secreted into nectar gathered by honeybees. Honey may be finally made up through the process whereby sucrose in nectar, in which glucose and fructose also are naturally contained, is hydrolyzed by secreted alpha-glucosidase III.
从欧洲蜜蜂（Apis mellifera L.）中纯化出了三种α-葡萄糖苷酶，即I、II和III。此外，还从蜂蜜中纯化出了一种α-葡萄糖苷酶。蜂蜜α-葡萄糖苷酶的一些特性，包括底物特异性，与α-葡萄糖苷酶III几乎相同。制备了针对这些α-葡萄糖苷酶的特异性抗血清，以检测α-葡萄糖苷酶在蜜蜂器官中的定位。首次通过免疫方法证实，α-葡萄糖苷酶I存在于中肠，α-葡萄糖苷酶II存在于中肠和血淋巴中。α-葡萄糖苷酶III显然就是蜂蜜α-葡萄糖苷酶，它存在于下咽腺中，该酶可能从这里分泌到蜜蜂采集的花蜜中。蜂蜜最终可能是通过花蜜中的蔗糖（其中天然也含有葡萄糖和果糖）被分泌的α-葡萄糖苷酶III水解的过程形成的。
