de Vries J, Wackernagel W
Fachbereich Biologie, Genetik, Universität Oldenburg, Germany.
J Gen Microbiol. 1992 Jan;138(1):31-8. doi: 10.1099/00221287-138-1-31.
In Escherichia coli, constituents of the main recombination pathway are provided by the genes recA (RecA protein) and recBCD (RecBCD enzyme). Recombination in conjugation experiments and repair of UV damage of E. coli mutants deleted for recA, for recBCD or for recA plus recBCD were restored, although to different degrees, by the cloned recA and recBCD genes from Serratia marcescens or Proteus mirabilis. When both recombination enzymes were from the same species, repair and recombination efficiencies had the order E. coli greater than S. marcescens greater than P. mirabilis. However, the P. mirabilis recA plus recBCD genes resulted in higher levels of repair and recombination than those obtained with one component from P. mirabilis (recA or recBCD) and the other from E. coli or S. marcescens. The data provide evidence for the similarity of RecABCD pathways of recombination among enteric bacteria and suggest an in vivo advantage of an intraspecies combination of P. mirabilis RecA protein and RecBCD enzyme over interspecies combinations. This could point to a cooperation between these basic recombination enzymes. The molecular processes which could be involved are discussed.
在大肠杆菌中，主要重组途径的组成成分由recA基因（RecA蛋白）和recBCD基因（RecBCD酶）提供。在接合实验中的重组以及recA、recBCD或recA加recBCD缺失的大肠杆菌突变体紫外线损伤的修复，通过来自粘质沙雷氏菌或奇异变形杆菌的克隆recA和recBCD基因得以恢复，尽管恢复程度不同。当两种重组酶来自同一物种时，修复和重组效率的顺序为大肠杆菌＞粘质沙雷氏菌＞奇异变形杆菌。然而，奇异变形杆菌的recA加recBCD基因所导致的修复和重组水平高于用奇异变形杆菌的一个组分（recA或recBCD）与大肠杆菌或粘质沙雷氏菌的另一个组分所获得的水平。这些数据为肠道细菌中RecABCD重组途径的相似性提供了证据，并表明奇异变形杆菌RecA蛋白和RecBCD酶的种内组合在体内比种间组合具有优势。这可能表明这些基本重组酶之间存在协同作用。文中讨论了可能涉及的分子过程。
