通过直接印刷工艺制造并带有末端通道安培检测的电泳微芯片。

Electrophoresis microchip fabricated by a direct-printing process with end-channel amperometric detection.

作者信息

Coltro Wendell K Tomazelli, da Silva José A Fracassi, da Silva Heron D Torres, Richter Eduardo M, Furlan Rogério, Angnes Lúcio, do Lago Claudimir L, Mazo Luiz H, Carrilho Emanuel

机构信息

Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos-SP, Brazil.

出版信息

Electrophoresis. 2004 Nov;25(21-22):3832-9. doi: 10.1002/elps.200406091.

DOI:10.1002/elps.200406091

PMID:15565680

Abstract

We describe the development of an electrophoresis microchip fabricated by a direct-printing process, based on lamination of printed polyester films with end-channel amperometric detection. The channel structures are defined by polyester (base and cover) and by a toner layer (walls). The polyester-toner devices presented an electroosmotic flow (EOF) magnitude of approximately 10(-5) cm2 V(-1) s(-1), which is generated by a polymeric mixture of the toner and polyester composition. The microelectrodes used for detection were produced combining this laser-printer technology to compact discs. The performance of this device was evaluated by amperometric detection of iodide and ascorbate. The detection limits found were 500 nmol.L(-1) (135 amol) and 1.8 micromol.L(-1) (486 amol) for iodide and ascorbate, respectively.

摘要

我们描述了一种通过直接印刷工艺制造的电泳微芯片的开发，该工艺基于带有末端通道安培检测的印刷聚酯薄膜层压。通道结构由聚酯（基底和盖）以及墨粉层（壁）定义。聚酯 - 墨粉装置呈现出约10^(-5) cm² V^(-1) s^(-1) 的电渗流（EOF）大小，这是由墨粉和聚酯成分的聚合物混合物产生的。用于检测的微电极是通过将这种激光打印机技术与光盘相结合而制成的。通过对碘化物和抗坏血酸盐的安培检测来评估该装置的性能。发现碘化物和抗坏血酸盐的检测限分别为500 nmol·L^(-1)（135 amol）和1.8 μmol·L^(-1)（486 amol）。