Siwicki K K, Schwartz W J, Hall J C
Department of Biology, Brandeis University, Waltham, Massachusetts 02254.
J Neurogenet. 1992 Feb;8(1):33-42. doi: 10.3109/01677069209167270.
Cell bodies in the rat suprachiasmatic nucleus (SCN) were labeled with an antibody against a small domain of the period (per) protein, the product of a gene in Drosophila that regulates circadian rhythms. In immunoblots of SCN protein extracts, the antibody recognized a band of approximately 115 kD, as well as a heterogeneous antigen ranging from 160 kD to 170 kD. The antibody was found in previous studies to label putative circadian pacemaker neurons in Aplysia and Bulla, as well as the cellular sites of per expression in flies. Taken together, these results suggest that the region of the per protein recognized by this antibody may be widely conserved in neuronal circadian pacemakers.
用一种针对周期蛋白（per）小结构域的抗体标记大鼠视交叉上核（SCN）中的细胞体，周期蛋白是果蝇中一个调节昼夜节律的基因的产物。在SCN蛋白提取物的免疫印迹中，该抗体识别出一条约115 kD的条带，以及一条分子量在160 kD至170 kD之间的异质性抗原条带。在之前的研究中发现，该抗体可标记海兔和泡螺中假定的昼夜节律起搏器神经元，以及果蝇中per表达的细胞位点。综合这些结果表明，该抗体识别的per蛋白区域可能在神经元昼夜节律起搏器中广泛保守。
