Aréchiga H, Rodríguez-Sosa L
División de Estudios de Posgrado e Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, DF, México.
Proc Biol Sci. 1998 Oct 7;265(1408):1819-23. doi: 10.1098/rspb.1998.0507.
Electrical mass response of crayfish photoreceptors (electroretinogram) was recorded continuously for up to seven days in isolated preparations that consisted of the retina and lamina ganglionaris. Electroretinogram amplitude varied in a circadian manner with a nocturnal acrophase and a period of 22-23 h in preparations kept in darkness. Acclimatization of animals to reversed light/dark cycles resulted in a phase reversal of the rhythm in vitro. The per (period) gene of Drosophila has been implicated in the genesis of rhythms in insects and in vertebrates. Immunocytochemical staining with an antibody against the PER gene product revealed immunoreactivity in the retinal photoreceptors, as well as in cell bodies in the lamina ganglionaris. Labelled axons run distally towards the photoreceptors and proximally to other areas of the lamina.
在由视网膜和视神经节层组成的离体标本中，连续记录小龙虾光感受器的电群体反应（视网膜电图）长达7天。在黑暗环境中的标本里，视网膜电图振幅呈昼夜节律变化，夜间出现峰值，周期为22 - 23小时。使动物适应颠倒的光/暗周期会导致体外节律的相位逆转。果蝇的per（周期）基因与昆虫和脊椎动物的节律产生有关。用抗PER基因产物的抗体进行免疫细胞化学染色显示，在视网膜光感受器以及视神经节层的细胞体中存在免疫反应性。标记的轴突向远端延伸至光感受器，向近端延伸至视神经节层的其他区域。
