Martin D S, Desser S S, Hong H
Department of Zoology, University of Toronto, Ontario, Canada.
J Parasitol. 1992 Apr;78(2):317-22.
Six metabolic enzymes, glucose-6-phosphate dehydrogenase, glucosephosphate isomerase, isocitrate dehydrogenase, malate dehydrogenase, phosphoglucomutase, and purine nucleoside phosphorylase, from clonal isolates of 3 presumptive species of Trypanosoma (T. fallisi, T. ranarum, and T. rotatorium) from 3 anuran hosts (Bufo americanus, Rana clamitans, and Rana catesbeiana) were compared using starch-gel electrophoresis. Although bands were shared among the different zymodemes of isolates of the same host genus, low genetic polymorphism of the enzyme loci was observed with few apparent shared bands between samples isolated from frogs and toads. A distance value calculated between toad and frog trypanosome isolates suggests the likelihood of long-time separation of species. Cluster analysis based on overall similarity distinguished the trypanosomes of toads and frogs as separate taxa, suggesting that host specificity and observed morphological differences are consistent with heritable allozyme differences.
利用淀粉凝胶电泳比较了来自3种无尾目宿主（美洲蟾蜍、绿蛙和牛蛙）的3种推测的锥虫物种（法氏锥虫、蛙锥虫和旋回锥虫）的克隆分离株中的6种代谢酶，即葡萄糖-6-磷酸脱氢酶、磷酸葡萄糖异构酶、异柠檬酸脱氢酶、苹果酸脱氢酶、磷酸葡萄糖变位酶和嘌呤核苷磷酸化酶。尽管同一宿主属分离株的不同酶带型之间存在共享条带，但观察到酶基因座的遗传多态性较低，从青蛙和蟾蜍分离的样本之间几乎没有明显的共享条带。蟾蜍和青蛙锥虫分离株之间计算出的距离值表明物种长期分离的可能性。基于总体相似性的聚类分析将蟾蜍和青蛙的锥虫区分为不同的分类群，这表明宿主特异性和观察到的形态差异与可遗传的等位酶差异一致。
