Li Tao, Walsh Jacob R, Ghishan Fayez K, Bai Liqun
Department of Pediatrics, Steele Memorial Children's Research Center, University of Arizona Health Sciences Center, Room 3325, 1501 North Campbell Avenue, Tucson, AZ 85724, USA.
Biochim Biophys Acta. 2004 Nov 24;1681(1):53-8. doi: 10.1016/j.bbaexp.2004.10.001.
We report the novel cloning and preliminary characterization of a human urate transporter (hURAT1) gene promoter. The transcription initiation site was mapped to a base 337 bp upstream of the ATG start codon by primer extension and 5'-RACE. The minimal functional promoter region is within 253 bp when the promoter/luciferase constructs were transfected into OK cells. The sex hormone testosterone significantly increases promoter activity, suggesting that hormonal regulation of hURAT1 is the root cause of observed differences in urate levels between males and females.
我们报告了人类尿酸转运蛋白（hURAT1）基因启动子的新型克隆及初步特性分析。通过引物延伸和5'-RACE技术，转录起始位点被定位到ATG起始密码子上游337 bp处的一个碱基。当将启动子/荧光素酶构建体转染到OK细胞中时，最小功能启动子区域在253 bp范围内。性激素睾酮显著增加启动子活性，这表明hURAT1的激素调节是观察到的男性和女性尿酸水平差异的根本原因。
