Clingenpeel Scott R, Keener William K, Keller Caron R, De Jesus Karl, Howard M Hope, Watwood Mary E
Department of Biological Sciences, Idaho State University, Pocatello, ID 83209, USA.
J Microbiol Methods. 2005 Jan;60(1):41-6. doi: 10.1016/j.mimet.2004.08.018.
3-Ethynylbenzoate (3EB) functions as a novel, activity-dependent, fluorogenic, and chromogenic probe for bacterial strains expressing the TOL pathway, which degrade toluene via conversion to benzoate, followed by meta ring fission of the intermediate catechol. This direct physiological analysis allows the fluorescent labeling of cells whose toluene-degrading enzymes have been induced by an aromatic substrate.
