Moore John E, Xu Jiru, Millar B Cherie
Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Lisburn Road, Belfast BT9 7AD, Northern Ireland, United Kingdom.
J Microbiol Methods. 2005 Jan;60(1):131-3. doi: 10.1016/j.mimet.2004.09.007.
Bacterial culture was allowed to dry to completeness on Columbia agar base with defibrinated horse blood. Following 6 months storage at room temperature, microbial DNA was extracted and successfully amplified by PCR. This storage technique has the advantage over other methods of not requiring (i) a DNA extraction protocol prior to storage and (ii) refrigeration and/or freezing. This technique maybe useful in the transportation of bacterial genomic DNA in nonviable cells as well as reliable method for the storage of DNA in underdeveloped countries.
将细菌培养物接种在含去纤维马血的哥伦比亚琼脂培养基上，使其完全干燥。在室温下储存6个月后，提取微生物DNA，并通过聚合酶链反应（PCR）成功扩增。与其他方法相比，这种储存技术的优势在于：（i）储存前无需DNA提取方案；（ii）无需冷藏和/或冷冻。该技术可能有助于在不发达国家非存活细胞中运输细菌基因组DNA，也是一种可靠的DNA储存方法。
