Pangas Stephanie A, Yan Wei, Matzuk Martin M, Rajkovic Aleksandar
Department of Pathology, Baylor College of Medicine, Houston, TX 77030, USA.
Gene Expr Patterns. 2004 Dec;5(2):257-63. doi: 10.1016/j.modgep.2004.07.008.
HORMA-domain containing proteins are involved in cell cycle regulation by interactions with chromatin. Through an in silico subtractive screen for mouse genes preferentially expressed in newborn ovaries, we identified a gene that would encode a novel mammalian HORMA domain-containing protein termed Newborn Ovary HORMA protein (Nohma). Reverse transcription polymerase chain reaction and Northern blot analysis have demonstrated a 1.7 kb transcript that is expressed exclusively in germ cells in both male and female mouse gonads. Nohma expression can be detected postnatally in male, and prenatally in female gonads, but shows a sexually dimorphic expression in adult gonads. The Nohma transcript is abundant in the adult testis but limited in the adult ovary. The mouse Nohma gene is comprised of 15 exons and maps to chromosome 3. A human ortholog was also identified. The expression pattern of Nohma suggests that it may be a critical regulatory protein in germ cell meiosis.
含HORMA结构域的蛋白质通过与染色质相互作用参与细胞周期调控。通过对优先在新生卵巢中表达的小鼠基因进行电子消减筛选，我们鉴定出一个基因，它编码一种新的含哺乳动物HORMA结构域的蛋白质，称为新生卵巢HORMA蛋白（Nohma）。逆转录聚合酶链反应和Northern印迹分析表明，存在一个1.7 kb的转录本，它仅在雄性和雌性小鼠性腺的生殖细胞中表达。Nohma表达在出生后可在雄性性腺中检测到，在雌性性腺中则在出生前可检测到，但在成年性腺中表现出性别二态性表达。Nohma转录本在成年睾丸中丰富，但在成年卵巢中有限。小鼠Nohma基因由15个外显子组成，定位于3号染色体。还鉴定出了人类同源基因。Nohma的表达模式表明它可能是生殖细胞减数分裂中的关键调节蛋白。
