毛发检测方法的比较研究。

A comparison study of hair examination methodologies.

作者信息

Kolowski Jason C, Petraco Nicholas, Wallace Margaret M, De Forest Peter R, Prinz Mechthild

机构信息

Science Department, John Jay College of Criminal Justice, City University of New York, 445 West 59th Street, New York, NY 10019, USA.

出版信息

J Forensic Sci. 2004 Nov;49(6):1253-5.

PMID:15568697

Abstract

A study was conducted to investigate the accuracy between two methods of hair analysis: PCR-STR DNA analysis and microscopic comparison analysis. Standard sets of pubic hairs were collected from volunteers, and unknown sets were generated from these samples. Three out of five (60%) of the hairs analyzed produced full DNA profiles that were correctly matched to the standard sets. DNA analysis was inconclusive (partial or no DNA profile) for two out of five (40%) of the samples. In contrast, the microscopic comparison analysis correctly matched four out of five (80%) of the samples to the standard sets but mis-identified one out of five (20%) of the samples. These results reinforce the practice of preliminary microscopic hair examination in narrowing down a set of hairs for DNA analysis. Microscopic comparison analysis is sufficiently reliable to remain a rapid and inexpensive method for forensic hair analysis.

摘要

开展了一项研究，以调查两种毛发分析方法的准确性：聚合酶链反应-短串联重复序列（PCR-STR）DNA分析和显微镜比较分析。从志愿者身上收集了标准的阴毛样本，并从这些样本中生成了未知样本集。在分析的五根毛发中，有三根（60%）产生了完整的DNA图谱，与标准样本集正确匹配。五分之二（40%）的样本DNA分析结果不明确（部分或无DNA图谱）。相比之下，显微镜比较分析将五分之四（80%）的样本与标准样本集正确匹配，但将五分之一（20%）的样本误识别。这些结果强化了在缩小用于DNA分析的毛发范围时进行初步显微镜毛发检查的做法。显微镜比较分析足够可靠，仍然是一种快速且廉价的法医毛发分析方法。