Pádár Z, Egyed B, Kontadakis K, Füredi S, Woller J, Zöldág L, Fekete S
DNA Laboratory, Institute for Forensic Sciences, P.O. Box 314/4, 1903 Budapest, Hungary.
Int J Legal Med. 2002 Oct;116(5):286-8. doi: 10.1007/s00414-002-0302-2. Epub 2002 May 24.
In a case of the death of a 7-year-old boy, the police investigations revealed a possible dog attack contrary to the witness testimonies. DNA investigations were carried out from hairs, saliva and bloodstains with 10 canine-specific STR loci by the use of fluorescently labelled multiplex PCR and the ABI PRISM 310 genetic analyzer. The analysis of one hair sample revealed one allele deviation from the profile of the putative Rottweiler perpetrator possibly caused by a mutation. The PCR fragments in question at the PEZ20 locus were sequenced and compared with the alleles detected in the Hungarian canine population and identified on a repeat number basis. The allele frequencies were determined based on typing of 242 genetically independent canine individuals from 72 breeds. The results suggested that two of the canine individuals could be the perpetrators.
在一名7岁男孩死亡的案件中，警方调查发现存在与证人证词相悖的可能的犬类袭击情况。利用荧光标记多重聚合酶链反应和ABI PRISM 310基因分析仪，对毛发、唾液和血迹进行了针对10个犬类特异性短串联重复序列位点的DNA调查。对一份毛发样本的分析显示，与推定的罗威纳犬肇事者的基因图谱存在一个等位基因偏差，这可能是由突变引起的。对PEZ20位点有问题的聚合酶链反应片段进行了测序，并与匈牙利犬类群体中检测到的等位基因进行比较，并根据重复数进行鉴定。等位基因频率是基于对来自72个品种的242个基因独立的犬类个体的分型确定的。结果表明，有两只犬类个体可能是肇事者。
