He Hai-Long, Wu Zhi-Hong, Zhang Jian-Guo, Wang Yi-Peng, Zhou Yan, Xu Ya-Qin, Yuan Jian-Gang, Qiu Gui-Xing
Orthopaedics Department of Peking Union Medical College Hospital, Beijing 100730, China.
Zhonghua Yi Xue Za Zhi. 2004 Oct 17;84(20):1681-5.
To study the distribution of collagen X in the intervertebral disc and determine its role in the pathogeny of idiopathic scoliosis (IS).
The data included apical disc and intermediate disc from 14 cases of AIS, 26 discs from 13 cases of scoliosis of confirmed pathogeny which included 10 cases of congenital scoliosis and neurofibromatosis scoliosis (CS group). Six discs were obtained from 3 cases of sudden death of normal young man served as controls. The distribution of collagen X in the apical disc of IS was examined by immunohistochemistry and in situ hybridization (ISH) with RNA probe. The figure of collagen X hybridization in the endplate cartilage was input to the figure analysis system. The mRNA content of collagen X was compared between every 2 groups by SPSS software.
Collagen X was mainly distributed around the hypertrophic chondrocyte in the endplate cartilage. Its mRNA was expressed in the hypertrophic chondrocyte. Positive signal was found in the nuclei of several scoliosis patients, which was not related to the pathogeny. The collagen X mRNA contents of the apical disc and intermediated disc of the IS group and of the CS group were significantly higher than those of the normal group (P < 0.05). There was no difference between the IS and CS groups.
Collagen X is mainly distributed around the hypertrophic chondrocyte in the endplate cartilage. Its can also be secreted by the chondrocyte-like cells in the nucleus under special condition. Higher expression of collagn X gene in scoliosis patients may be the effects of long term abnormal stress which causes calcification of endplate cartilage. Collagen X expressed in the nucleus may be the result of secretion of chondrocyte-like cells in the disc under abnormal mechanical condition.
研究Ⅹ型胶原蛋白在椎间盘的分布情况，并确定其在特发性脊柱侧凸（IS）发病机制中的作用。
数据包括14例青少年特发性脊柱侧凸（AIS）患者的终板椎间盘和中间椎间盘，13例明确病因的脊柱侧凸患者的26个椎间盘，其中包括10例先天性脊柱侧凸和神经纤维瘤病性脊柱侧凸（CS组）。从3例正常青年猝死患者获取6个椎间盘作为对照。采用免疫组织化学和RNA探针原位杂交（ISH）检测IS患者终板椎间盘内Ⅹ型胶原蛋白的分布。将终板软骨中Ⅹ型胶原蛋白杂交图像输入图像分析系统。运用SPSS软件比较每两组之间Ⅹ型胶原蛋白的mRNA含量。
Ⅹ型胶原蛋白主要分布于终板软骨肥大软骨细胞周围。其mRNA在肥大软骨细胞中表达。在部分脊柱侧凸患者的细胞核中发现阳性信号，这与发病机制无关。IS组和CS组终板椎间盘和中间椎间盘的Ⅹ型胶原蛋白mRNA含量均显著高于正常组（P<0.05）。IS组和CS组之间无差异。
Ⅹ型胶原蛋白主要分布于终板软骨肥大软骨细胞周围。在特殊情况下，其也可由细胞核内的类软骨细胞分泌。脊柱侧凸患者中Ⅹ型胶原蛋白基因的高表达可能是长期异常应力导致终板软骨钙化的结果。细胞核中表达的Ⅹ型胶原蛋白可能是椎间盘在异常力学条件下类软骨细胞分泌的结果。
