从“高危”血清阴性受试者体外产生HIV-1特异性抗体。
In vitro production of HIV-1 specific antibodies from "at risk" seronegative subjects.
作者信息
Quarto M, Fiore J R, Chironna M, Fontana A, Germinario C, Troiano T, Fico C, Congedo P, Grottola A, Monno L
机构信息
Institute of Hygiene, University of Bari, Italy.
出版信息
Microbiologica. 1992 Jan;15(1):29-33.
We investigated a group of well characterized seronegative subjects "at risk" for HIV-1 infection including heterosexual partners of HIV-1 infected subjects and intravenous drug abusers. Pokeweed mitogen (PWM)-stimulated peripheral blood mononuclear cells (PBMCs) were studied for their ability to produce antibodies against HIV-1 structural proteins in vitro. Viral activity by means of HIV-1 isolation from PBMCs and presence of serum p24 antigen were also tested. In 7/42 cases (16.6%) HIV-1 immunoreactive specific antibodies were found, mostly directed against the envelope proteins (gp 120/160). Remarkably, none of these in vitro antibody producers yielded HIV-1 isolation in cell cultures or had detectable serum levels of p24 antigen.
我们研究了一组具有明确特征的血清学阴性且有感染HIV-1“风险”的受试者，包括HIV-1感染者的异性伴侣和静脉吸毒者。研究了美洲商陆丝裂原（PWM）刺激的外周血单个核细胞（PBMC）在体外产生抗HIV-1结构蛋白抗体的能力。还检测了通过从PBMC中分离HIV-1来测定的病毒活性以及血清p24抗原的存在情况。在42例中的7例（16.6%）中发现了HIV-1免疫反应性特异性抗体，主要针对包膜蛋白（gp 120/160）。值得注意的是，这些体外抗体产生者中没有一人在细胞培养中分离出HIV-1，也没有可检测到的血清p24抗原水平。
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