Filice G, Cereda P M, Orsolini P, Soldini L, Romero E, Rondanelli E G
Department of Infectious Diseases, University of Pavia, Italy.
Microbiologica. 1990 Jul;13(3):215-24.
In rabbits experimentally infected with 1.10(5) u.i./ml HIV, IgM antibodies were detected 10-15 days after infection, reaching peak value two weeks later and remaining stable for two weeks long. Then a the IgM serotiters progressively decreased and were negative at ten weeks. HIV p24 antigen was detected ten-fifteen days after infection, reaching peak value five-six weeks later. Antigenemia subsequently decreased and reached a second peak after nine weeks. In our experimental conditions, the antigenemia persisted throughout the observation period. The IgG antibody titer reached a maximum two weeks after infection; the time course showed a decrease after ten weeks, followed by progressively decreasing fluctuating course. After twenty four weeks of infection the serotiter values though lower were always positive. Three-four weeks after infection we detected IgG antibodies to the major core protein p24. Reactivity of IgG antibodies to gp41 was observed earlier than reactivity to p24; these antibodies were detected over six months after infection. Viruses indistinguishable from HIV were isolated from the peripheral blood mononuclear cells of infected rabbits 30, 60 and 180 days after infection. These data further confirm that the rabbit may serve as an economical and reproducible model for HIV infection in which vaccines and antiviral agents could be tested.
在实验感染1.10(5) 国际单位/毫升HIV的兔子中，感染后10 - 15天检测到IgM抗体，两周后达到峰值，并持续稳定两周。然后IgM血清滴度逐渐下降，十周时呈阴性。HIV p24抗原在感染后十至十五天被检测到，五至六周后达到峰值。随后抗原血症下降，并在九周后出现第二个峰值。在我们的实验条件下，抗原血症在整个观察期持续存在。IgG抗体滴度在感染后两周达到最高值；时间进程显示十周后下降，随后呈逐渐下降的波动过程。感染二十四周后，血清滴度值虽较低但始终为阳性。感染后三至四周，我们检测到针对主要核心蛋白p24的IgG抗体。IgG抗体对gp41的反应性比其对p24的反应性出现得更早；这些抗体在感染后六个月以上被检测到。在感染后30、60和180天，从感染兔子的外周血单核细胞中分离出与HIV无法区分的病毒。这些数据进一步证实兔子可作为一种经济且可重复的HIV感染模型，用于测试疫苗和抗病毒药物。
